Herbicidal Active Compound Ferulic Acid Ethyl Ester Affects Fatty Acid Synthesis by Targeting the 3-Ketoacyl-Acyl Carrier Protein Synthase I (KAS I),Journal of Agricultural and Food Chemistry

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Herbicidal Active Compound Ferulic Acid Ethyl Ester Affects Fatty Acid Synthesis by Targeting the 3-Ketoacyl-Acyl Carrier Protein Synthase I (KAS I)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-01-02 , DOI: 10.1021/acs.jafc.2c07214
Shujie Ma ₁ , Ran Jia ₁ , Xin Li ₁ , Wen Wang ₁ , Liyu Jin ₁ , Xinxin Zhang ₁ , Hualong Yu ₁ , Juan Yang ₂ , Lili Dong ₁ , Lihui Zhang ₁ , Jingao Dong ₁

Affiliation

Exploring new herbicide targets based on natural product derivatives is an important research aspect for the generation of innovative pesticides. Ferulic acid ethyl ester (FAEE), a natural product derivative from ferulic acid, has significant herbicidal activity mainly by inhibiting the normal growth of weed seedling roots. However, the FAEE target protein underlying its herbicidal activity has not been identified. In this study, we synthesized an FAEE probe to locate its site of action. We discovered that FAEE entry point was via the root tips. Fourteen major binding proteins were identified using Drug affinity responsive target stability (DARTS) combined with LC-MS/MS, which included 3-ketoacyl-acyl carrier protein synthase I (KAS I) and phenylalanine ammonia-lyase I (PAL I). The KAS I and PAL I proteins/genes expression was changed significantly after exposure to FAEE, as evidenced by combined transcriptomic and proteomic analysis. A molecular docking assay indicated that KAS I and FAEE had a strong binding ability. Combined with previous studies on FAEE mechanism of action, and based on our results, we conclude that FAEE targeting KAS I lead to the blockage of the fatty acid synthesis pathway and result in plant death.

中文翻译：

除草活性化合物阿魏酸乙酯通过靶向 3-酮酰基-酰基载体蛋白合成酶 I (KAS I) 影响脂肪酸合成

基于天然产物衍生物探索除草新靶点是创新农药产生的重要研究方向。阿魏酸乙酯(FAEE)是阿魏酸的天然产物衍生物，主要通过抑制杂草幼苗根系的正常生长而具有显着的除草活性。然而，尚未确定其除草活性背后的 FAEE 靶蛋白。在这项研究中，我们合成了一个 FAEE 探针来定位其作用位点。我们发现 FAEE 入口点是通过根提示。使用药物亲和反应靶稳定性 (DARTS) 结合 LC-MS/MS 鉴定了 14 种主要结合蛋白，其中包括 3-酮酰基-酰基载体蛋白合成酶 I (KAS I) 和苯丙氨酸解氨酶 I (PAL I)。KAS I 和 PAL I 蛋白/基因表达在暴露于 FAEE 后发生显着变化，如转录组学和蛋白质组学联合分析所证明的那样。分子对接分析表明，KAS I 和 FAEE 具有很强的结合能力。结合以往对 FAEE 作用机制的研究，并根据我们的结果，我们得出结论，针对 KAS I 的 FAEE 会导致脂肪酸合成途径受阻并导致植物死亡。

更新日期：2023-01-02

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