三分法则：控制 3D DNA 拼块中的结手性和极性,Small

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三分法则：控制 3D DNA 拼块中的结手性和极性
Small ( IF 13.0 ) Pub Date : 2022-12-30 , DOI: 10.1002/smll.202206511
Simon Vecchioni ₁ , Brandon Lu ₁ , Jordan Janowski ₁ , Karol Woloszyn ₁ , Nataša Jonoska ₂ , Nadrian C Seeman ₁ , Chengde Mao ₃ , Yoel P Ohayon ₁ , Ruojie Sha ₁

Affiliation

张拉整体三角形 DNA 晶体的成功自组装预示着能够从合理设计的纳米级组件中以可编程方式构建宏观晶体纳米材料。这个 3D DNA 瓦片的“张拉整体”性质归功于它的三个旋转堆叠的双螺旋，它们通过中心链的拉伸缠绕锁定在一起，中心链被分成 7 个碱基对 (bp) 的连接区域，对应于三分之二的螺旋圈脱氧核糖核酸。迄今为止所有报告的张拉整体三角形都采用

(Z + 2个 / 3个) \[\left( {Z{\bm{ + }}2{\bf /}3} \right)\]

转动交叉路口段，产生右手反平行“J1”交叉路口。这里报告了一个最小的 DNA 三角形基序，它由 3-bp 的连接间片段组成，或三分之一的螺旋转角。研究发现，最小基序呈现出由局部平行霍利迪连接点（“L1”连接点）介导的左手三级结构的反转形态。这种平行连接产生了一个预测的螺旋槽匹配模式，打破了瓷砖面之间的伪对称性，连接形态进一步表明了一种折叠机制。确定了三分法则，通过该法则可以通过连接点间 DNA 片段长度对超分子手性进行编程。

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The Rule of Thirds: Controlling Junction Chirality and Polarity in 3D DNA Tiles

The successful self-assembly of tensegrity triangle DNA crystals heralded the ability to programmably construct macroscopic crystalline nanomaterials from rationally-designed, nanoscale components. This 3D DNA tile owes its “tensegrity” nature to its three rotationally stacked double helices locked together by the tensile winding of a center strand segmented into 7 base pair (bp) inter-junction regions, corresponding to two-thirds of a helical turn of DNA. All reported tensegrity triangles to date have employed

(Z + 2 / 3) \[\left( {Z{\bm{ + }}2{\bf /}3} \right)\]

turn inter-junction segments, yielding right-handed, antiparallel, “J1” junctions. Here a minimal DNA triangle motif consisting of 3-bp inter-junction segments, or one-third of a helical turn is reported. It is found that the minimal motif exhibits a reversed morphology with a left-handed tertiary structure mediated by a locally-parallel Holliday junction—the “L1” junction. This parallel junction yields a predicted helical groove matching pattern that breaks the pseudosymmetry between tile faces, and the junction morphology further suggests a folding mechanism. A Rule of Thirds by which supramolecular chirality can be programmed through inter-junction DNA segment length is identified. These results underscore the role that global topological forces play in determining local DNA architecture and ultimately point to an under-explored class of self-assembling, chiral nanomaterials for topological processes in biological systems.

更新日期：2022-12-30

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