Nanozyme with the merit of excellent and adjustable catalytic activity, outstanding stability and low cost is a promising alternative for natural enzymes widely applied in a variety of fields. In the present study, a new two-dimensional cobalt-based MOF nanocomposite designated as MVCM@β-CD was synthesized. Combined with the strategies of increasing the ratio of Co(Ⅲ)/Co(Ⅱ) and modifying with small molecule β-cyclodextrin (β-CD), MVCM@β-CD displayed remarkably enhanced oxidase-mimicking activity, which was attributed to synergistic effect from large surface area of two dimensional Co-MOF nanosheet, numerous exposed active sites, high-proportioned trivalence of cobalt and regulating action of β-cyclodextrin. The addition of aminophenol isomers inhibited the catalytic oxidation process, resulting in different color change of the solution and UV–Vis absorption behaviors, based on which a sensitive ratiometric colorimetry for m-aminophenol (m-Ap) and a simple colorimetric p-aminophenol (p-Ap) detection method were developed with the detection limit of 0.16 μM and 1.01 μM, respectively. This method realized the colorimetric differentiation of aminophenol isomers, which provided a simple, accurate and low-cost approach for visual discrimination without complicated instrument and procedure, especially appropriate for on-site detection.

纳米酶具有催化活性优异且可调、稳定性好、成本低等优点，是一种很有前途的天然酶替代品，广泛应用于各个领域。在本研究中，合成了一种新的二维钴基 MOF 纳米复合材料，命名为 MVCM@β-CD。结合提高Co(Ⅲ)/Co(Ⅱ)的比例和用小分子β-环糊精（β-CD）修饰的策略，MVCM@β-CD显示出显着增强的氧化酶模拟活性，这归因于协同作用二维Co-MOF纳米片的大表面积，大量暴露的活性位点，钴的高比例三价和β-环糊精的调节作用。氨基苯酚异构体的加入抑制了催化氧化过程，建立了间氨基苯酚（m -Ap）和简单的比色法对氨基苯酚（p -Ap）检测方法，检测限分别为0.16 μM和1.01 μM。该方法实现了氨基酚同分异构体的比色区分，为视觉判别提供了一种简便、准确、低成本的方法，无需复杂的仪器和程序，特别适用于现场检测。