Effect and mechanism of safranal on ISO-induced myocardial injury based on network pharmacology,Journal of Ethnopharmacology

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Effect and mechanism of safranal on ISO-induced myocardial injury based on network pharmacology
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2022-12-29 , DOI: 10.1016/j.jep.2022.116103
Meijuan Yan ₁ , Jichuan Zhao ₁ , Yingjie Kang ₂ , Luqian Liu ₁ , Wenjun He ₁ , Yufang Xie ₂ , Rui Wang ₂ , Liya Shan ₂ , Xinzhi Li ₁ , Ketao Ma ₂

Affiliation

Ethnopharmacological relevance

Sympathetic hyperactivation is a significant risk factor in the development of cardiovascular disease. Safranal has shown good myocardial protection in recent studies, but the mechanism of its role in myocardial injury caused by sympathetic hyperactivation remains unclear.

Aim of the study

The purpose of this study was to investigate whether safranal can effectively reduce isoproterenol (ISO)-induced myocardial injury in rats and H9c2 cells and to reveal its pharmacological action and target in inhibiting myocardial injury caused by sympathetic hyperactivation.

Materials and methods

This study was carried out using network pharmacology, molecular docking, and in vitro and in vivo experiments. An in vivo model of myocardial injury was established by subcutaneous injection of ISO, and an in vitro model of H9c2 cell injury was induced by ISO.

Results

Safranal ameliorated myocardial injury caused by sympathetic hyperactivation by reducing the level of myocardial apoptosis. According to the results of network pharmacological analysis and molecular docking, the mechanism by which safranal alleviates myocardial injury may be closely related to the TNF signaling pathway, and safranal plays a role by regulating the core targets of the TNF signaling pathway. Safranal significantly inhibited the protein expression of TNF, PTGS2, MMP9 and pRELA.