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Efficient Synthetic Aperture for Phaseless Fourier Ptychographic Microscopy with Hybrid Coherent and Incoherent Illumination
Laser & Photonics Reviews ( IF 9.8 ) Pub Date : 2022-12-27 , DOI: 10.1002/lpor.202200201
Yao Fan 1, 2, 3 , Jiasong Sun 1, 2, 3 , Yefeng Shu 1, 2, 3 , Zuxin Zhang 1, 2, 3 , Guoan Zheng 4 , Wenjian Chen 1, 2, 3 , Jin Zhang 5 , Kun Gui 6 , Kehui Wang 6 , Qian Chen 1, 2, 3 , Chao Zuo 1, 2, 3
Laser & Photonics Reviews ( IF 9.8 ) Pub Date : 2022-12-27 , DOI: 10.1002/lpor.202200201
Yao Fan 1, 2, 3 , Jiasong Sun 1, 2, 3 , Yefeng Shu 1, 2, 3 , Zuxin Zhang 1, 2, 3 , Guoan Zheng 4 , Wenjian Chen 1, 2, 3 , Jin Zhang 5 , Kun Gui 6 , Kehui Wang 6 , Qian Chen 1, 2, 3 , Chao Zuo 1, 2, 3
Affiliation
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Fourier ptychographic microscopy (FPM) is a computational high-throughput technique for high-resolution and wide field-of-view (FOV) imaging applications such as cell biology, medicine screening, and digital pathology. By integrating angle-varied illumination, iteration phase retrieval, and synthetic aperture, it achieves a significantly enhanced imaging resolution beyond the diffraction limit of the objective lens while retaining its original large FOV without any mechanical movement. However, sufficient data redundancy is a prerequisite for the convergence of the iteration algorithm, which in turn requires dozens or even hundreds of raw images to get a decent resolution, leaving ample room for further improvement. In this paper, an efficient synthetic aperture scheme for FPM is proposed, termed ESA-FPM. It employs both coherent and incoherent illuminations to maximize the efficiency of data utilization and achieve high spatial-bandwidth product (SBP) reconstruction with few acquisitions. The data redundancy requirements are further analyzed, suggesting that ESA-FPM reaches imaging resolution of using only seven images. The experiment with USAF target demonstrates that ESA-FPM achieves theoretical resolution with only 1.6% of the data of conventional FPM. A customized miniaturized ESA-FPM system with a high-numerical-aperture, low-magnification objective lens, and a high-brightness LED array is built, demonstrating its potential for biomedical and pathological applications.
中文翻译:
具有混合相干和非相干照明的无相傅立叶印刷显微镜的高效合成孔径
傅里叶叠层显微术 (FPM) 是一种计算高通量技术,适用于高分辨率和宽视场 (FOV) 成像应用,例如细胞生物学、医学筛选和数字病理学。通过集成变角照明、迭代相位检索和合成孔径,它实现了超越物镜衍射极限的显着增强的成像分辨率,同时保持其原始的大 FOV,无需任何机械移动。然而,足够的数据冗余是迭代算法收敛的前提,而迭代算法又需要数十甚至数百张原始图像才能获得不错的分辨率,从而为进一步改进留下了充足的空间。在本文中,提出了一种有效的 FPM 合成孔径方案,称为 ESA-FPM。它采用相干和非相干照明来最大限度地提高数据利用效率,并通过少量采集实现高空间带宽积 (SBP) 重建。进一步分析了数据冗余要求,表明 ESA-FPM 达到了成像分辨率仅使用七张图片。美国空军目标的实验表明,ESA-FPM 仅用传统 FPM 的 1.6% 的数据就达到了理论分辨率。构建了具有高数值孔径、低放大倍率物镜和高亮度 LED 阵列的定制微型 ESA-FPM 系统,展示了其在生物医学和病理学应用中的潜力。
更新日期:2022-12-27
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
具有混合相干和非相干照明的无相傅立叶印刷显微镜的高效合成孔径
傅里叶叠层显微术 (FPM) 是一种计算高通量技术,适用于高分辨率和宽视场 (FOV) 成像应用,例如细胞生物学、医学筛选和数字病理学。通过集成变角照明、迭代相位检索和合成孔径,它实现了超越物镜衍射极限的显着增强的成像分辨率,同时保持其原始的大 FOV,无需任何机械移动。然而,足够的数据冗余是迭代算法收敛的前提,而迭代算法又需要数十甚至数百张原始图像才能获得不错的分辨率,从而为进一步改进留下了充足的空间。在本文中,提出了一种有效的 FPM 合成孔径方案,称为 ESA-FPM。它采用相干和非相干照明来最大限度地提高数据利用效率,并通过少量采集实现高空间带宽积 (SBP) 重建。进一步分析了数据冗余要求,表明 ESA-FPM 达到了成像分辨率仅使用七张图片。美国空军目标的实验表明,ESA-FPM 仅用传统 FPM 的 1.6% 的数据就达到了理论分辨率。构建了具有高数值孔径、低放大倍率物镜和高亮度 LED 阵列的定制微型 ESA-FPM 系统,展示了其在生物医学和病理学应用中的潜力。