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Inhibition and Removal of Mature Mixed-Bacteria Biofilms on Voice Prostheses by Sodium Selenite
Infection and Drug Resistance ( IF 2.9 ) Pub Date : 2022-12-29 , DOI: 10.2147/idr.s393434 Yongli Zhang 1, 2 , Yanyan Niu 1, 2 , Hong Huo 1, 2 , Jian Wang 1, 2 , Xiaofeng Jin 1, 2 , Hua Yang 1, 2
Infection and Drug Resistance ( IF 2.9 ) Pub Date : 2022-12-29 , DOI: 10.2147/idr.s393434 Yongli Zhang 1, 2 , Yanyan Niu 1, 2 , Hong Huo 1, 2 , Jian Wang 1, 2 , Xiaofeng Jin 1, 2 , Hua Yang 1, 2
Affiliation
Purpose: Biofilms on voice prostheses are important factors shortening their service life. Sodium selenite has been used to prevent and treat various diseases. Whether sodium selenite can inhibit and remove mature biofilms on voice prostheses is still unknown.
Methods: To verify the effects of sodium selenite on mature mixed-bacteria biofilms (Staphylococcus aureus, Candida albicans, and Streptococcus faecalis) on voice prostheses, we used quantitative and qualitative methods, eg, real-time fluorescence quantitative PCR, crystal violet staining, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (XTT) reduction assays, and scanning electron microscopy, to measure the effects of sodium selenite on the number of bacterial colonies, biofilm formation ability, metabolic activity, and ultrastructure in a model of mature mixed-bacteria biofilms on voice prostheses and validated the effects in vitro on mature biofilms on voice prostheses from patients.
Results: When exploring the possible mechanism of biofilm inhibition and removal by sodium selenite, we found that it significantly inhibited and removed biofilms on voice prostheses and effectively destroyed the spatial structure of the biofilms. The inhibition and removal effects became more significant with increasing sodium selenite concentrations.
Conclusion: We demonstrated that sodium selenite can inhibit and remove biofilms of mature mixed strains on voice prostheses, providing a novel basis for treating patients’ voice prosthesis biofilms.
Keywords: sodium selenite, voice prostheses, mature biofilms, mixed strains on voice prostheses, remove, inhibit
中文翻译：
亚硒酸钠对人工发音体成熟混合菌生物膜的抑制和去除作用
目的：发音假体上的生物膜是缩短其使用寿命的重要因素。亚硒酸钠已被用于预防和治疗多种疾病。亚硒酸钠能否抑制和去除人工发音体上的成熟生物膜尚不清楚。
方法：验证亚硒酸钠对成熟混合菌生物膜（金黄色葡萄球菌、白色念珠菌和粪链球菌）的影响) 在语音假肢上，我们使用了定量和定性的方法，例如实时荧光定量PCR、结晶紫染色、2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5- carboxanilide) (XTT) 还原试验和扫描电子显微镜，以测量亚硒酸钠对人工发音器官成熟混合细菌生物膜模型中细菌菌落数量、生物膜形成能力、代谢活性和超微结构的影响，并经过验证体外对成熟生物膜对患者发音假体的影响。
结果：在探索亚硒酸钠抑制和去除生物膜的可能机制时，我们发现它能显着抑制和去除人工发音体上的生物膜，并有效破坏生物膜的空间结构。随着亚硒酸钠浓度的增加，抑制和去除效果更加显着。
结论：我们证明了亚硒酸钠可以抑制和去除人工发音成熟混合菌株的生物膜，为治疗患者人工发音生物膜提供了新的依据。
关键词：亚硒酸钠，人工发音，成熟生物膜，人工发音混合菌株，去除，抑制
更新日期：2022-12-29
Methods: To verify the effects of sodium selenite on mature mixed-bacteria biofilms (Staphylococcus aureus, Candida albicans, and Streptococcus faecalis) on voice prostheses, we used quantitative and qualitative methods, eg, real-time fluorescence quantitative PCR, crystal violet staining, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (XTT) reduction assays, and scanning electron microscopy, to measure the effects of sodium selenite on the number of bacterial colonies, biofilm formation ability, metabolic activity, and ultrastructure in a model of mature mixed-bacteria biofilms on voice prostheses and validated the effects in vitro on mature biofilms on voice prostheses from patients.
Results: When exploring the possible mechanism of biofilm inhibition and removal by sodium selenite, we found that it significantly inhibited and removed biofilms on voice prostheses and effectively destroyed the spatial structure of the biofilms. The inhibition and removal effects became more significant with increasing sodium selenite concentrations.
Conclusion: We demonstrated that sodium selenite can inhibit and remove biofilms of mature mixed strains on voice prostheses, providing a novel basis for treating patients’ voice prosthesis biofilms.
Keywords: sodium selenite, voice prostheses, mature biofilms, mixed strains on voice prostheses, remove, inhibit
中文翻译：
亚硒酸钠对人工发音体成熟混合菌生物膜的抑制和去除作用
目的：发音假体上的生物膜是缩短其使用寿命的重要因素。亚硒酸钠已被用于预防和治疗多种疾病。亚硒酸钠能否抑制和去除人工发音体上的成熟生物膜尚不清楚。
方法：验证亚硒酸钠对成熟混合菌生物膜（金黄色葡萄球菌、白色念珠菌和粪链球菌）的影响) 在语音假肢上，我们使用了定量和定性的方法，例如实时荧光定量PCR、结晶紫染色、2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5- carboxanilide) (XTT) 还原试验和扫描电子显微镜，以测量亚硒酸钠对人工发音器官成熟混合细菌生物膜模型中细菌菌落数量、生物膜形成能力、代谢活性和超微结构的影响，并经过验证体外对成熟生物膜对患者发音假体的影响。
结果：在探索亚硒酸钠抑制和去除生物膜的可能机制时，我们发现它能显着抑制和去除人工发音体上的生物膜，并有效破坏生物膜的空间结构。随着亚硒酸钠浓度的增加，抑制和去除效果更加显着。
结论：我们证明了亚硒酸钠可以抑制和去除人工发音成熟混合菌株的生物膜，为治疗患者人工发音生物膜提供了新的依据。
关键词：亚硒酸钠，人工发音，成熟生物膜，人工发音混合菌株，去除，抑制
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