Phloretin(PRT), phlorizin(PRZ), and ferulic acid(FEA) prevalent in apples are unstable and less soluble in water, which can be improved by cyclodextrin(CD) encapsulation. This study aimed to provide atomistic insights of β-CD–PRT (1), β-CD–PRZ (2), and α-CD–FEA (3) complexes. Single-crystal X-ray diffraction (XRD) revealed that one PRZ (2) and one FEA (3) insert the aromatic B-ring and C=C–C=O(O) group respectively into the β-CD (2) and α-CD (3) cavities, whereas a half-occupied PRT (1) inserts the B-ring across the β-CD cavity. The induced-fit process yielded thermodynamically stable complexes 2 > 1 > 3, in agreement with the density functional theory (DFT)-optimized structures with the corresponding number of intermolecular OH∙∙∙O H-bonds (7 > 3 > 1). Perpendicular conformations of the pharmaceutically active forms of PRT (1) and PRZ (2) are first observed crystallographically. This study confirmed the potential applications of CDs as molecular stabilizers and aqueous solubilizers for the improved bioavailability and efficient delivery of food bioactive compounds.

Chemical compounds studied in this article:

α-Cyclodextrin (PubChem CID: 444913); β-Cyclodextrin (PubChem CID: 444041); Ferulic acid (PubChem CID: 445858); Phloretin (PubChem CID: 4788); Phlorizin (PubChem CID: 6072); Ethanol (PubChem CID: 702)