通过多域 DNA 结构模拟 Titin,ACS Macro Letters

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通过多域 DNA 结构模拟 Titin
ACS Macro Letters ( IF 5.1 ) Pub Date : 2022-12-27 , DOI: 10.1021/acsmacrolett.2c00585
Nanpu Cao ₁ , Wanhao Cai ₁ , Lu Qian ₁ , Zhou Nie ₂ , Chengde Mao ₃ , Shuxun Cui ₁

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Titin 是一种包含多个串联结构域的巨型蛋白质，对于维持肌肉的卓越机械性能至关重要。域的连续和可逆展开和重新折叠对于 titin 作为模块化弹簧至关重要。自从发现单个 titin 分子的机械特性以来，探索具有模拟 titin 模块化结构的仿生材料一直是一个活跃的领域。然而，由于复杂的合成过程，大规模制备这些模块化聚合物仍然是一个挑战。在这项研究中，我们建议将具有多个发夹的模块化 DNA (MH-DNA) 作为高级材料自下而上设计的基本模块。通过基于原子力显微镜 (AFM) 的单分子力谱 (SMFS) 分析模块化发夹的展开和重新折叠动力学，我们发现 MH-DNA 显示出与肌联蛋白等多蛋白相当的稳定性。模块化发夹独特的低滞后性使其成为理想的分子弹簧，具有卓越的机械效率。在成熟的 DNA 合成技术的基础上，我们预计 MH-DNA 可以用作先进材料的有前途的构建块，具有优异的结构稳定性、可观的可扩展性和高机械效率。

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Emulating Titin by a Multidomain DNA Structure

Titin, a giant protein containing multiple tandem domains, is essential in maintaining the superior mechanical performance of muscle. The consecutive and reversible unfolding and refolding of the domains are crucial for titin to serve as a modular spring. Since the discovery of the mechanical features of a single titin molecule, the exploration of biomimetic materials with titin-emulating modular structures has been an active field. However, it remains a challenge to prepare these modular polymers on a large scale due to the complex synthesis process. In this study, we propose modular DNA with multiple hairpins (MH-DNA) as the fundamental block for the bottom-up design of advanced materials. By analyzing the unfolding and refolding dynamics of modular hairpins by atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS), we find that MH-DNA shows comparable stability to those of polyproteins like titin. The unique low hysteresis of modular hairpin makes it an ideal molecular spring with remarkable mechanical efficiency. On the basis of the well-established DNA synthesis techniques, we anticipate that MH-DNA can be used as a promising building block for advanced materials with a combination of superior structural stability, considerable extensibility, and high mechanical efficiency.

更新日期：2022-12-27

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