Due to growing health concerns, the urban population is utterly inclined towards a healthy lifestyle and incorporated nutritional food supplements to lower common health risks. The ω-3 and ω-6 PUFAs consumption is increasing, hence alternative commercial production is essentially developed. The microbial source is an emerging platform to overcome the global demand for omega PUFAs. Marine oleaginous protist Aurantiochytrium sp. found a potential source to produce substantial DHA and SFA. The objective of the present research was to enhance the PUFA yield by optimizing maximum tolerable glucose concentration with a suitable nitrogen ratio (10:1). The maximum lipid and DHA yield and content were determined 4.30, 1.34 g/L, and 62.4, 33.49 % of total biomass and lipid at 30 g/L glucose respectively, which is one of among highest reported, however relative PUFA was maximum 46.97 % (DHA) in total lipid at 10 g/L glucose. Remaining 42–53.6 % SFA could be used for biodiesel.

由于对健康的关注日益增加，城市人口完全倾向于健康的生活方式，并加入营养食品补充剂以降低常见的健康风险。ω-3 和 ω-6 PUFAs 的消耗量正在增加，因此替代商业生产基本上得到发展。微生物源是一个新兴平台，可以满足全球对欧米茄多不饱和脂肪酸的需求。海洋产油原生生物Aurantiochytrium sp。找到了生产大量 DHA 和 SFA 的潜在来源。本研究的目的是通过优化最大可耐受葡萄糖浓度和合适的氮比 (10:1) 来提高 PUFA 产量。在 30 g/L 葡萄糖下，脂质和 DHA 的最大产量和含量分别为 4.30、1.34 g/L 和 62.4、33.49 % 的总生物量和脂质，这是报道的最高之一，但相对 PUFA 最高为 46.97 % (DHA) 在 10 g/L 葡萄糖的总脂质中。剩余的 42–53.6% SFA 可用于生产生物柴油。