淀粉葡萄糖苷酶和普鲁兰酶对燕麦淀粉的水解,Starch

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淀粉葡萄糖苷酶和普鲁兰酶对燕麦淀粉的水解
Starch ( IF 2.6 ) Pub Date : 2022-12-25 , DOI: 10.1002/star.202200201
Esra Başarıcı Ünlü ₁ , Çiğdem Aykaç ₁

Affiliation

本研究描述了在实验室条件下将燕麦淀粉酶促转化为麦芽糖和葡萄糖。首先，通过两种不同的提取方法（碱法和酸碱法）分离燕麦淀粉。其次，对两种提取方法获得的 10% 和 12.5% 淀粉溶液进行酶促淀粉水解。第三，研究了糖化过程中支链淀粉酶与淀粉葡萄糖苷酶的使用情况。最后，对提取方法、淀粉浓度和普鲁兰酶使用的影响进行了评估。使用通过酸碱法获得的 12.5% 淀粉溶液导致淀粉转化为葡萄糖和麦芽糖的转化率更高，与碱淀粉相比表现出更好的糖浆生产底物。支链淀粉酶的使用增加了淀粉转化为葡萄糖和麦芽糖的速率。当普鲁兰酶 (12 U) 与淀粉葡萄糖苷酶一起使用时，发现淀粉转化为葡萄糖的最高转化率为 49.06%。最高葡萄糖当量（DE）值为97.71，是在10%酸碱淀粉中用支链淀粉酶得到的。研究中获得的数据表明，燕麦淀粉可以成为糖浆生产的合适来源。12.5% 的淀粉浓度提供了高产率，普鲁兰酶的使用增加了水解产物的量和 DE 值。

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Hydrolysis of Oat Starch by Amyloglucosidase and Pullulanase

This study describes the enzymatic conversion of oat starch to maltose and glucose under laboratory conditions. First, oat starch is isolated by two different extraction methods (alkali and acid-alkali). Second, enzymatic starch hydrolysis is carried out for 10% and 12.5% starch solutions obtained by both extraction methods. Third, pullulanase usage together with amyloglucosidase is investigated during saccharification. Finally, effects of the extraction method, starch concentration, and use of pullulanase are evaluated. The use of a 12.5% starch solution obtained by the acid-alkali method results in higher rates of conversion of starch to glucose and maltose, expressing better substrate for syrup production compared to alkali starch. The use of pullulanase increases the rate of starch conversion to glucose and maltose. The highest conversion of starch to glucose is found to be 49.06% when pullulanase (12 U) is used with amyloglucosidase. The highest dextrose equivalence (DE) value is 97.71, and it is obtained by using pullulanase in 10% acid-alkali starch. The data obtained in the study show that oat starch can be a suitable source for use in sugar syrup production. The 12.5% starch concentration provides high yield, and the use of pullulanase increases both the amount of hydrolysis products and the DE value.

更新日期：2022-12-25

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