Hepcidin antimicrobial peptide (hamp) is active in teleosts against invading pathogens and plays important roles in the stress and immune responses of finfish. The response of hamp gene was studied in yellow perch (yp) (Perca flavescens) challenged with lipopolysaccharides to understand if this immunity response is sex-specifically different. The cloned hamp gene consists of an open-reading frame of 273 bp and encodes a deduced protein of 90 amino acids (a.a.), which includes a signal peptide of 24 a.a., a pro-domain of 40 a.a. and a mature peptide of 26 a.a. Yp hamp involves 8 cysteine residues with 4 disulfide bonds, and a protein with an internal alpha helix flanked with C- and N-terminal random coils was modeling predicted. RT-qPCR was used to analyze the relative abundances (RAs) of hamp mRNA in the livers of juvenile female and male yellow perch challenged with lipopolysaccharide. The expression levels of hamp were significantly elevated by 3 h (RA = 7.3) and then peaked by 6 h (RA = 29.4) post-treatment in females but the peak was delayed to 12 h (RA = 65.4) post-treatment in males. The peak mRNA level of challenged males was shown 7.6-fold higher than females. The post-treatment responses in both genders decreased to their lowest levels by 24 h and 48 h. Overall, female perch had an earlier but less-sensitive response to the lipopolysaccharide challenge than male.

铁调素抗菌肽 ( hamp ) 在硬骨鱼中具有抗入侵病原体的活性，并在有鳍鱼的应激和免疫反应中发挥重要作用。在用脂多糖攻击的黄鲈鱼 (yp) ( Perca flavescens ) 中研究了hamp基因的反应，以了解这种免疫反应是否存在性别特异性差异。克隆的hamp基因由一个273 bp的开放阅读框组成，编码一个90个氨基酸（aa）的推导蛋白，包括24个aa的信号肽、40个aa的前域和26个aa的成熟肽。 Yp汉普涉及具有 4 个二硫键的 8 个半胱氨酸残基，并且建模预测了一种蛋白质，该蛋白质具有侧翼为 C 和 N 末端随机卷曲的内部 α 螺旋。RT-qPCR 用于分析脂多糖攻击的幼年雌性和雄性黄鲈肝脏中hamp mRNA 的相对丰度 (RAs)。hamp的表达水平显着升高 3 小时 (RA = 7.3)，然后在女性治疗后 6 小时 (RA = 29.4) 达到峰值，但男性治疗后峰值延迟至 12 小时 (RA = 65.4)。受攻击雄性的 mRNA 峰值水平比雌性高 7.6 倍。两种性别的治疗后反应均在 24 小时和 48 小时时降至最低水平。总体而言，雌性鲈鱼对脂多糖挑战的反应比雄性更早但不那么敏感。