本研究采用超声辅助提取法从藏红花柱头中提取生物活性化合物。通过使用响应面方法 (RSM) 优化工艺参数。将甲醇百分比 (0–100%)、超声处理持续时间 (分钟) 和不同的保持温度 (°C) 保持为独立变量，以优化藏红花素、苦藏红花素和藏红花醛这三种藏红花的主要成分的提取。采用高效液相色谱法 (HPLC) 评估藏红花提取物中藏红花素、苦藏红花素和藏红花醛的浓度。最终数据包括四种模型：线性、线性平方、线性相互作用和完全二次。与R 2crocin、picrocrocin 和 safranal 的值分别为 0.90、0.92 和 0.80，因此确定完全二次模型是最有效的。响应面分析表明，提取藏红花素、苦味苦素和番红花醛的理想条件分别为甲醇浓度为 52.59、超声处理时间为 4.34 分钟和保温温度为 68.78 °C。藏红花素、苦藏红花素和藏红花醛含量的 E1cm%1 λmax 经验量分别为 523.531、2656.59 和 11,035.61。在超声辅助提取条件下，优化提取物的总酚含量 (103.063 ± 0.00 mg GAE/100 g) 和总黄酮含量 (98.25 ± 0.01 mg QE/100 g) 高于总酚含量 (94.311 ± 0.00 mg GAE) /100 g) 和总黄酮含量 (92.210 ± 0. 02 mg QE/100 g) 常规提取样品 (CES)。在优化的超声提取样品中观察到比传统提取样品 (80.00%) 更高的 DPPH 活性 (88.08%)。我们的数据显示，超声波辅助提取提高了这些有价值的生物活性成分的回收率；中等甲醇浓度、高温和短持续时间对生物活性成分提取效率有显着影响。我们关于萃取条件的发现与工业和分析应用相关。中等甲醇浓度、高温和短持续时间对生物活性成分提取效率有显着影响。我们关于萃取条件的发现与工业和分析应用相关。中等甲醇浓度、高温和短持续时间对生物活性成分提取效率有显着影响。我们关于萃取条件的发现与工业和分析应用相关。
"点击查看英文标题和摘要"
Response surface methodology (RSM)-based statistical modeling and optimization of the ultrasound-assisted extraction of saffron bioactives
The present study was carried out for the extraction of bioactive compounds from saffron stigma using the ultrasound-assisted extraction. Process parameters were optimized by using response surface methodology (RSM). The percentage of methanol (0–100%), duration of sonication (min), and varied holding temperatures (°C) were kept as independent variables to optimize the extraction of crocin, picrocrocin, and safranal, three main components of saffron. High-performance liquid chromatography (HPLC) was employed to assess the concentration of crocin, picrocrocin, and safranal in saffron extract. The final data included four models: linear, linear squares, linear interactions, and complete quadratic. With R2 values of 0.90, 0.92, and 0.80 for crocin, picrocrocin, and safranal, respectively, the full quadratic model was determined to be the most effective. Response surface analysis revealed that the ideal conditions for the extraction of crocin, picrocin, and safranal were methanol concentration of 52.59, sonication time of 4.34 min, and holding temperature of 68.78 °C respectively. The empirical quantities of E1cm%1 λmax for crocin, picrocrocin, and safranal, contents were 523.531, 2656.59, and 11,035.61 respectively. The total phenolic content (103.063 ± 0.00 mg GAE/100 g) and total flavonoid content (98.25 ± 0.01 mg QE/100 g) of optimized extracts were higher under ultrasound-assisted extraction conditions than the total phenolic content (94.311 ± 0.00 mg GAE/100 g) and total flavonoid content (92.210 ± 0.02 mg QE/100 g) of conventionally extracted samples (CES). The higher DPPH activity (88.08%) was observed in the optimized ultrasonically extracted sample than in conventionally extracted samples (80.00%). Our data revealed that ultrasound-assisted extraction increased the recovery of these valuable bioactive components; moderate methanol concentrations, high temperatures, and short durations had a remarkable influence on bioactive component extraction efficiency. Our discoveries regarding extraction conditions are relevant for both industrial and analytical applications.
京公网安备 11010802027423号