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Paper-based multi-well depletion ELISA
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-12-22 , DOI: 10.1039/d2lc00960a Dohwan Lee 1 , Norh Asmare 1 , A Fatih Sarioglu 1, 2, 3
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-12-22 , DOI: 10.1039/d2lc00960a Dohwan Lee 1 , Norh Asmare 1 , A Fatih Sarioglu 1, 2, 3
Affiliation
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Enzyme-linked immunosorbent assay (ELISA) is widely employed for detecting target molecules in bioassays including the serological assays that measure specific antibody titers. However, ELISA tests are inherently limited to centralized laboratories staffed with trained personnel as the assay workflow requires multiple steps to be performed in a specific sequence. Here, we report a dipstick ELISA test that automates this otherwise laborious process and reports the titer of a target molecule in a digital manner without the need for an external instrument or operator. Our assay measures titer by gradually immuno-depleting the target analyte from a flowing sample effectively diluting the residual target – a process conventionally achieved through serially diluting the whole sample in numerous, time-consuming pipetting steps performed manually. Furthermore, the execution of the depletion ELISA process is automated by a built-in flow controller which sequentially delivers different reagents with preset delays. We apply the technology to develop assays measuring (1) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody titers (IgM/IgG antibodies to nucleocapsid and spike protein) and (2) troponin I, a cardiac biomarker.
中文翻译：
纸基多孔耗尽 ELISA
酶联免疫吸附测定 (ELISA) 广泛用于检测生物测定中的目标分子，包括测量特定抗体滴度的血清学测定。然而，ELISA 测试本质上仅限于配备训练有素人员的集中实验室，因为化验工作流程需要按特定顺序执行多个步骤。在这里，我们报告了一种试纸 ELISA 测试，它可以自动执行这个原本费力的过程，并以数字方式报告目标分子的滴度，而无需外部仪器或操作员。我们的测定通过逐渐免疫耗尽流动样本中的目标分析物来有效稀释残留目标来测量滴度——这一过程通常是通过手动执行大量耗时的移液步骤连续稀释整个样本来实现的。此外，耗尽 ELISA 过程的执行由内置流量控制器自动执行，该控制器按预设延迟顺序输送不同的试剂。我们应用该技术开发检测方法，用于测量 (1) 严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 抗体滴度（针对核衣壳和刺突蛋白的 IgM/IgG 抗体）和 (2) 肌钙蛋白 I，一种心脏生物标志物。
更新日期：2022-12-22
中文翻译：
纸基多孔耗尽 ELISA
酶联免疫吸附测定 (ELISA) 广泛用于检测生物测定中的目标分子，包括测量特定抗体滴度的血清学测定。然而，ELISA 测试本质上仅限于配备训练有素人员的集中实验室，因为化验工作流程需要按特定顺序执行多个步骤。在这里，我们报告了一种试纸 ELISA 测试，它可以自动执行这个原本费力的过程，并以数字方式报告目标分子的滴度，而无需外部仪器或操作员。我们的测定通过逐渐免疫耗尽流动样本中的目标分析物来有效稀释残留目标来测量滴度——这一过程通常是通过手动执行大量耗时的移液步骤连续稀释整个样本来实现的。此外，耗尽 ELISA 过程的执行由内置流量控制器自动执行，该控制器按预设延迟顺序输送不同的试剂。我们应用该技术开发检测方法，用于测量 (1) 严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 抗体滴度（针对核衣壳和刺突蛋白的 IgM/IgG 抗体）和 (2) 肌钙蛋白 I，一种心脏生物标志物。
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