精氨酸-甘氨酸-天冬氨酸和磷脂酰丝氨酸对钛种植体表面免疫调节和骨整合的协同作用,Biomaterials Science

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精氨酸-甘氨酸-天冬氨酸和磷脂酰丝氨酸对钛种植体表面免疫调节和骨整合的协同作用
Biomaterials Science ( IF 5.8 ) Pub Date : 2022-12-22 , DOI: 10.1039/d2bm01589g
Lele Wu ₁ , Gyeung Mi Seon ₁ , Sungwon Ju ₁ , Sang Hoon Choi ₁ , En-Shi Jiang ₁ , Yongjoon Kim ₁ , Shin Hye Chung ₁ , Jin-Soo Ahn ₁ , Hyeong-Cheol Yang ₁

Affiliation

巨噬细胞极化的控制在骨整合等骨组织再生中很重要。在这项研究中，开发了一种涂层方法，通过产生免疫调节作用来改善钛 (Ti) 植入物的骨整合。Ti 圆盘的表面涂有聚（丙交酯-共-乙交酯）(PLGA) 聚合物、磷脂酰丝氨酸 (PS) 和精氨酸-甘氨酸-天冬氨酸 (RGD) 肽偶联磷脂。在体外使用小鼠骨髓源性巨噬细胞 (BMDM) 进行的测定，M2 标记基因（Arg-1、YM-1、FIZZ1）和 CD206（一种 M2 表面标记）的最显着表达是通过含有 6 mol% RGD 偶联物的涂层获得的和由 50 mol% PS 组成的磷脂。RGD 和 PS 的 M2 诱导作用也在大鼠股骨中得到验证，其中植入了涂层钛棒。RGD 和 PS 涂层显着增强了 Ti 种植体的骨整合。此外，生物力学推出试验表明，RGD 和 PS 涂层增加了骨与植入物之间的界面结合力。这些结果表明，PS 和 RGD 可以应用于可植入生物医学装置的固体表面，以改善免疫调节和组织再生。

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Synergistic effects of arginine–glycine–aspartic acid and phosphatidylserine on the surface immunomodulation and osseointegration of titanium implants

The control of macrophage polarization is important in bone tissue regeneration such as osseointegration. In this study, a coating method was developed to improve the osseointegration of titanium (Ti) implants by generating an immunomodulatory effect. The surface of the Ti discs was coated with a poly(lactide-co-glycolide)(PLGA) polymer, phosphatidylserine (PS), and arginine–glycine–aspartic acid (RGD) peptide conjugated phospholipid. In in vitro assay using mouse bone marrow-derived macrophages (BMDMs), the most significant expression of the M2 marker genes (Arg-1, YM-1, FIZZ1) and CD206, an M2 surface marker, was obtained with coatings containing 6 mol% RGD conjugates and phospholipids consisting of 50 mol% PS. The M2-inducing effect of RGD and PS was also verified in rat femurs where coated Ti rods were implanted. The RGD and PS coating significantly enhanced the osseointegration of the Ti implants. Moreover, a biomechanical push-out test showed that the RGD and PS coating increased the interfacial binding force between the bone and implants. These results indicate that PS and RGD can be applied to the solid surface of implantable biomedical devices to improve immunomodulation and tissue regeneration.

更新日期：2022-12-22

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