Alkaline Phosphatase-Mediated Bioetching of CoOOH/BiVO4 for Signal-On Organic Photoelectrochemical Transistor Bioanalysis,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Alkaline Phosphatase-Mediated Bioetching of CoOOH/BiVO4 for Signal-On Organic Photoelectrochemical Transistor Bioanalysis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-12-20 , DOI: 10.1021/acs.analchem.2c04447
Rui Ban _{1,

2} , Cheng-Jun Li _{1,

2,

3} , Yi-Tong Xu ₃ , Yu-Yue Zhu _{3,

4} , Peng Ju ₄ , Yu-Mei Li ₂ , Hai-Jun Du ₂ , Jin Hu _{3,

5} , Guangxu Chen ₆ , Peng Lin ₅ , Wei-Wei Zhao ₃

Affiliation

Organic photoelectrochemical transistor (OPECT) bioanalytics has recently appeared as a promising route for biological measurements, which has major implications in both next-generation photoelectrochemical (PEC) bioanalysis and futuristic biorelated implementations. Via biological dissociation of materials, bioetching is a useful technique for bio-manufacturing and bioanalysis. The intersection of these two domains is expected to be a possible way to achieve innovative OPECT bioanalytics. Herein, we validate such a possibility, which is exemplified by alkaline phosphatase (ALP)-mediated bioetching of a CoOOH/BiVO₄ gate for a signal-on OPECT immunoassay of human immunoglobulin G (HIgG) as the model target. Specifically, target-dependent bioetching of the upper CoOOH layer could result into an enhanced electrolyte contact and light accessibility to BiVO₄, leading to the modulated response of the polymeric poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel that could be monitored by the channel current. The introduced biosensor achieves sensitive detection of HIgG with high selectivity and sensitivity. This work features bioetching-enabled high-efficacy OPECT bioanalysis and is anticipated to serve as a generic protocol, considering the diverse bioetching routes.

中文翻译：

碱性磷酸酶介导的 CoOOH/BiVO4 生物蚀刻用于信号有机光电化学晶体管生物分析

有机光电化学晶体管 (OPECT) 生物分析最近作为一种有前途的生物测量途径出现，对下一代光电化学 (PEC) 生物分析和未来生物相关实施具有重要意义。通过材料的生物解离，生物蚀刻是一种用于生物制造和生物分析的有用技术。这两个领域的交叉有望成为实现创新 OPECT 生物分析的可能途径。在此，我们验证了这种可能性，例如碱性磷酸酶 (ALP) 介导的 CoOOH/BiVO _{4生物蚀刻}以人免疫球蛋白 G (HIgG) 作为模型靶标的 OPECT 免疫测定的信号门。具体而言，上层 CoOOH 层的目标依赖性生物蚀刻可导致增强的电解质接触和光对 BiVO ₄的可及性，从而导致聚合物聚（3,4-乙烯二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT： PSS）通道，可以通过通道电流进行监控。引入的生物传感器以高选择性和灵敏度实现了 HIgG 的灵敏检测。这项工作具有支持生物蚀刻的高效 OPECT 生物分析，预计将作为通用协议，考虑到不同的生物蚀刻路线。

更新日期：2022-12-20

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南