Chicken intestine as a poultry industry byproduct is rich in protein, contains various endogenous enzymes and can be prepared as a hydrolysate via autolysis. This research examined the physicochemical properties of chicken hydrolysate as a feed attractant. Growth of fish fed on the chicken hydrolysate-coated diet was compared with fish fed on a commercial hydrolysate (C). Three chicken hydrolysates were prepared at 60 °C for 4 h on a pilot plant scale including autolysis at pH 3 (S1), supplemental enzyme addition at pH 6.11 (S2) and a combination of supplemental enzyme addition following autolysis at pH 3 (S3). Highest yield was found in S2 (76%) followed by S3 (74%) and S1 (72%). The chicken hydrolysate had lower protein content (13–16%), higher fat (21–23%) and lower viscosity at 7429–10607 cP compared with C. Degrees of hydrolysis (DH) of S1, S2, S3 and C were 56%, 73%, 65% and 61%, respectively. Highest number of peptides less than 3 kDa associated with feed palatability was found in S2 (76%), followed by S3 (49%), C (29%) and S1 (28%). The three chicken hydrolysates were rich in essential amino acids similar to the C sample but with smaller amounts of amino acids in S1 and S3 due to the acidic pH preparation. An 8-week feeding trial of two selected fish species (hybrid catfish and Nile tilapia) was conducted in a water-recirculation system to investigate the effect of different hydrolysate-coated diets on fish growth performance. Highest body weight of hybrid catfish at 65.20 g gave weight gain of 150.15% and specific growth rate 1.73% d⁻¹. Optimal feed conversion ratio (FCR) at 1.49 was observed in the S2 diet (p < 0.05). Improved growth performance was observed in Nile tilapia for diets coated with all chicken hydrolysates but no significant difference was found (p ≥ 0.05). Highest values of the hematological parameters, including hemoglobin, hematocrit and mean corpuscular hemoglobin concentration, were found in the two fish groups fed with S1, S2 and S3 diets (p < 0.05). At the end of the experiment, hybrid catfish and Nile tilapia fed with the S2 and S3 diets had the highest fish body protein content at 17% and 16%, respectively (p < 0.05). Our results showed that the three chicken hydrolysates, particularly S2, could potentially be used as feed attractants for freshwater fish.

鸡肠作为家禽业的副产品，富含蛋白质，含有多种内源性酶，可通过自溶制备为水解物。本研究考察了鸡水解物作为饲料引诱剂的理化特性。将喂食鸡水解物涂层饮食的鱼的生长与喂食商业水解物 (C) 的鱼进行比较。在试验工厂规模上，在 60 °C 下制备三种鸡肉水解物 4 小时，包括在 pH 3 下自溶 (S1)、在 pH 6.11 下添加补充酶 (S2) 以及在 pH 3 下自溶后添加补充酶的组合 (S3) . S2 (76%) 的产量最高，其次是 S3 (74%) 和 S1 (72%)。与 C 相比，鸡肉水解物具有较低的蛋白质含量 (13–16%)、较高的脂肪 (21–23%) 和较低的粘度（7429–10607 cP）。S1 的水解度 (DH)，S2、S3和C分别为56%、73%、65%和61%。在 S2 (76%) 中发现与饲料适口性相关的小于 3 kDa 的肽数量最多，其次是 S3 (49%)、C (29%) 和 S1 (28%)。三种鸡肉水解物富含与 C 样品相似的必需氨基酸，但由于酸性 pH 制备，S1 和 S3 中的氨基酸含量较少。在水循环系统中对两种选定的鱼类（杂交鲶鱼和尼罗罗非鱼）进行了为期 8 周的饲养试验，以研究不同的水解物涂层日粮对鱼类生长性能的影响。杂交鲶鱼的最高体重为 65.20 g，增重为 150.15%，比生长率为 1.73% d 在 S2 (76%) 中发现与饲料适口性相关的小于 3 kDa 的肽数量最多，其次是 S3 (49%)、C (29%) 和 S1 (28%)。三种鸡肉水解物富含与 C 样品相似的必需氨基酸，但由于酸性 pH 制备，S1 和 S3 中的氨基酸含量较少。在水循环系统中对两种选定的鱼类（杂交鲶鱼和尼罗罗非鱼）进行了为期 8 周的饲养试验，以研究不同的水解物涂层日粮对鱼类生长性能的影响。杂交鲶鱼的最高体重为 65.20 g，增重为 150.15%，比生长率为 1.73% d 在 S2 (76%) 中发现与饲料适口性相关的小于 3 kDa 的肽数量最多，其次是 S3 (49%)、C (29%) 和 S1 (28%)。三种鸡肉水解物富含与 C 样品相似的必需氨基酸，但由于酸性 pH 制备，S1 和 S3 中的氨基酸含量较少。在水循环系统中对两种选定的鱼类（杂交鲶鱼和尼罗罗非鱼）进行了为期 8 周的饲养试验，以研究不同的水解物涂层日粮对鱼类生长性能的影响。杂交鲶鱼的最高体重为 65.20 g，增重为 150.15%，比生长率为 1.73% d 三种鸡肉水解物富含与 C 样品相似的必需氨基酸，但由于酸性 pH 制备，S1 和 S3 中的氨基酸含量较少。在水循环系统中对两种选定的鱼类（杂交鲶鱼和尼罗罗非鱼）进行了为期 8 周的饲养试验，以研究不同的水解物涂层日粮对鱼类生长性能的影响。杂交鲶鱼的最高体重为 65.20 g，增重为 150.15%，比生长率为 1.73% d 三种鸡肉水解物富含与 C 样品相似的必需氨基酸，但由于酸性 pH 制备，S1 和 S3 中的氨基酸含量较少。在水循环系统中对两种选定的鱼类（杂交鲶鱼和尼罗罗非鱼）进行了为期 8 周的饲养试验，以研究不同的水解物涂层日粮对鱼类生长性能的影响。杂交鲶鱼的最高体重为 65.20 g，增重为 150.15%，比生长率为 1.73% d^-1。在 S2 日粮中观察到的最佳饲料转化率 (FCR) 为 1.49 ( p  < 0.05)。在尼罗罗非鱼中观察到用所有鸡水解物包衣的饮食改善了生长性能，但未发现显着差异 ( p  ≥ 0.05)。在喂食 S1、S2 和 S3 日粮的两个鱼组中发现了血液学参数的最高值，包括血红蛋白、血细胞比容和平均红细胞血红蛋白浓度 ( p  < 0.05)。实验结束时，饲喂 S2 和 S3 日粮的杂交鲶鱼和尼罗罗非鱼的鱼体蛋白含量最高，分别为 17% 和 16%（p < 0.05)。我们的结果表明，三种鸡水解物，尤其是 S2，有可能用作淡水鱼的饲料引诱剂。