胰岛素受体底物 1 (IRS1) 通过介导 FOXO 转录因子表达，独立于 JH 和 20E 信号通路，调节 Propylea japonica 的卵子发生和卵黄发生,Journal of Agricultural and Food Chemistry

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胰岛素受体底物 1 (IRS1) 通过介导 FOXO 转录因子表达，独立于 JH 和 20E 信号通路，调节 Propylea japonica 的卵子发生和卵黄发生
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-12-20 , DOI: 10.1021/acs.jafc.2c07433
Ningbo Huangfu _{1,

2} , Xiangzhen Zhu ₂ , Li Wang ₂ , Kaixin Zhang ₂ , Dongyang Li ₂ , Lulu Chen ₃ , Xueke Gao _{1,

2} , Lin Niu ₂ , Mengxue Gao ₂ , Jichao Ji _{1,

2} , Junyu Luo _{1,

2} , Jinjie Cui _{1,

2}

Affiliation

胰岛素受体底物 (IRS) 作为胰岛素/胰岛素样信号转导 (IIS) 通路中的核心细胞质衔接蛋白，是细胞信号转导的重要介质。然而，IRS 与激素信号的串扰如何调节昆虫的生长、发育和繁殖仍然是未知的。在这项研究中，我们证明IRS1的敲低显着抑制卵子发生、卵黄发生以及护士细胞和卵泡上皮细胞的发育。此外，qRT-PCR 结果表明，FOXO转录因子对IRS1基因的沉默有显着反应。然而，IRS1沉默对保幼激素/20-羟基蜕皮激素 (JH/20E) 信号基因、JH 合成和降解酶相关基因的表达以及 JH/20E 滴度没有显着影响。我们的结果表明 IIS 通路通过FOXO调节卵巢发育和Vg产生，独立于 JH 和 20E 信号通路。该研究揭示了大花海棠的繁殖调控机制，为大花海棠作为环境友好型生物防治策略的大规模推广提供了理论依据。

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Insulin Receptor Substrate-1 (IRS1) Regulates Oogenesis and Vitellogenesis in Propylea japonica by Mediating the FOXO Transcription Factor Expression, Independent of JH and 20E Signaling Pathways

The insulin receptor substrate (IRS), as the core cytoplasmic adapter protein in the insulin/insulin-like signaling (IIS) pathway, is an important mediator of cellular signaling. However, it is still unknown how IRS crosstalk with hormone signaling regulates insect growth, development, and reproduction. In this study, we demonstrated that knockdown of IRS1 significantly inhibited oogenesis, vitellogenesis, and the development of nurse cells and follicular epithelial cells. In addition, qRT-PCR results showed that FOXO transcription factors significantly responded to silencing of the IRS1 gene. However, IRS1 silencing had no significant effect on the expression of juvenile hormone/20-hydroxyecdysone (JH/20E)-signaling genes, JH synthesis, and degradation enzyme-related genes and the JH/20E titers. Our results suggested that the IIS pathway regulated ovarian development and Vg production through FOXO, independent of JH and 20E signaling pathways. This study revealed the reproductive regulation mechanism in Propylea japonica, which provides a theoretical basis for large-scale expansion of P. japonica as an environment-friendly biological control strategy.

更新日期：2022-12-20

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