胆固醇氧化酶固定化 MXene/海藻酸钠/二氧化硅@正二十二烷分级微胶囊用于胆固醇的超灵敏电化学生物传感检测,Journal of Materials Chemistry B

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胆固醇氧化酶固定化 MXene/海藻酸钠/二氧化硅@正二十二烷分级微胶囊用于胆固醇的超灵敏电化学生物传感检测
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2022-12-19 , DOI: 10.1039/d2tb02367a
Meng Zhang ₁ , Huan Liu ₁ , Xiaodong Wang ₁

Affiliation

电化学生物传感器在高温环境下通常会由于蛋白质变性和酶等生物识别元件失活而导致检测灵敏度和测定精度下降。着眼于有效解决这一关键问题，我们开发了胆固醇氧化酶固定化 MXene/海藻酸钠/二氧化硅@正二十二烷分级微胶囊作为电化学生物传感器的温度调节电极材料，以满足高温超灵敏检测胆固醇的要求。微胶囊首先通过微囊化n-二十二烷作为二氧化硅壳中的相变材料 (PCM)，然后沉积生物相容性海藻酸钠层，用电活性 MXene 纳米片包裹，然后固定胆固醇氧化酶作为电化学生物传感的生物识别元件。制造的复合材料不仅表现出具有所需化学和生物成分的逐层分层微结构，而且还通过其 PCM 核心的可逆相变获得超过 133 J g -1 的高潜热容量用于热管理^。用开发的复合材料对裸露的玻碳电极进行修饰，以用作胆固醇生物传感器。这使得修饰的电极能够获得原位温度调节能力调节电极周围的微环境温度，有效防止胆固醇氧化酶的蛋白质变性，最大限度地减少热对生物传感性能的影响。与没有PCM的传统胆固醇生物传感器相比，开发的生物传感器在高温下实现了4.63 μA μM ⁻¹ cm ⁻²的更高灵敏度和0.081 μM的检测下限，为真实生物样品提供了高精度和可靠的胆固醇检测在很宽的温度范围内。

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Cholesterol oxidase-immobilized MXene/sodium alginate/silica@n-docosane hierarchical microcapsules for ultrasensitive electrochemical biosensing detection of cholesterol

Electrochemical biosensors usually suffer from the deterioration of detection sensitivity and determination accuracy in a high-temperature environment due to protein denaturation and inactivation of their biological recognition elements such as enzymes. Focusing on an effective solution to this crucial issue, we have developed cholesterol oxidase-immobilized MXene/sodium alginate/silica@n-docosane hierarchical microcapsules as a thermoregulatory electrode material for electrochemical biosensors to meet the requirement of ultrasensitive detection of cholesterol at high temperature. The microcapsules were first fabricated by microencapsulating n-docosane as a phase change material (PCM) in a silica shell, followed by depositing a biocompatible sodium alginate layer, wrapping with electroactive MXene nanosheets and then immobilizing cholesterol oxidase as a biological recognition element for electrochemical biosensing. The fabricated composites not only exhibited a layer-by-layer hierarchical microstructure with the desired chemical and biological components, but also obtained a high latent-heat capacity of over 133 J g⁻¹ for thermal management through reversible phase transitions of its PCM core. A bare glassy carbon electrode was modified with the developed composites to serve for the cholesterol biosensor. This enables the modified electrode to obtain an in situ thermoregulatory ability to regulate the microenvironmental temperature surrounding the electrode, effectively preventing the protein denaturation of cholesterol oxidase and minimizing heat impact on biosensing performance. Compared to conventional cholesterol biosensors without a PCM, the developed biosensor achieved a higher sensitivity of 4.63 μA μM⁻¹ cm⁻² and a lower limit of detection of 0.081 μM at high temperature, providing highly accurate and reliable detection of cholesterol for real biological samples over a wide temperature range.

更新日期：2022-12-19

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