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The Wsp chemosensory system modulates c-di-GMP-dependent biofilm formation by integrating DSF quorum sensing through the WspR-RpfG complex in Lysobacter
npj Biofilms and Microbiomes ( IF 7.8 ) Pub Date : 2022-12-16 , DOI: 10.1038/s41522-022-00365-1
Kangwen Xu 1, 2 , Limin Wang 1 , Dan Xiong 1 , Hongjun Chen 3 , Xinru Tong 3 , Xiaolong Shao 1 , Tao Li 3 , Guoliang Qian 1
Affiliation  

The ubiquitous Wsp (wrinkly spreader phenotype) chemosensory system and DSF (diffusible signal factor) quorum sensing are two important chemically associated signaling systems that mediate bacterial communications between the host and environment. Although these two systems individually control biofilm formation in pathogenic bacteria via the ubiquitous second messenger c-di-GMP, their crosstalk mechanisms remain elusive. Here we present a scenario from the plant-beneficial and antifungal bacterium Lysobacter enzymogenes OH11, where biofilm formation favors the colonization of this bacterium in fungal hyphae. We found that the Wsp system regulated biofilm formation via WspR-mediated c-di-GMP signaling, whereas DSF system did not depend on the enzymatic activity of RpfG to regulate biofilm formation. We further found that WspR, a diguanylate cyclase (DGC) responsible for c-di-GMP synthesis, could directly bind to one of the DSF signaling components, RpfG, an active phosphodiesterase (PDE) responsible for c-di-GMP degradation. Thus, the WspR-RpfG complex represents a previously undiscovered molecular linker connecting the Wsp and DSF systems. Mechanistically, RpfG could function as an adaptor protein to bind and inhibit the DGC activity of unphosphorylated WspR independent of its PDE activity. Phosphorylation of WspR impaired its binding affinity to RpfG and also blocked the ability of RpfG to act as an adaptor protein, which enabled the Wsp system to regulate biofilm formation in a c-di-GMP-dependent manner by dynamically integrating the DSF system. Our findings demonstrated a previously uncharacterized mechanism of crosstalk between Wsp and DSF systems in plant-beneficial and antifungal bacteria.



中文翻译:

Wsp 化学感应系统通过 Lysobacter 中的 WspR-RpfG 复合物整合 DSF 群体感应来调节 c-di-GMP 依赖性生物膜的形成

无处不在的 Wsp(皱纹传播表型)化学感应系统和 DSF(扩散信号因子)群体感应是两个重要的化学相关信号系统,可介导宿主与环境之间的细菌通讯。尽管这两个系统通过无处不在的第二信使 c-di-GMP 分别控制病原菌中生物膜的形成,但它们的串扰机制仍然难以捉摸。在这里,我们展示了一种来自植物有益和抗真菌细菌Lysobacter enzymogenes的情景OH11,其中生物膜的形成有利于这种细菌在真菌菌丝中的定殖。我们发现 Wsp 系统通过 WspR 介导的 c-di-GMP 信号调节生物膜形成,而 DSF 系统不依赖于 RpfG 的酶活性来调节生物膜形成。我们进一步发现,负责 c-di-GMP 合成的二鸟苷酸环化酶 (DGC) WspR 可以直接与 DSF 信号成分之一 RpfG 结合,RpfG 是一种负责 c-di-GMP 降解的活性磷酸二酯酶 (PDE)。因此,WspR-RpfG 复合体代表了以前未发现的连接 Wsp 和 DSF 系统的分子接头。从机制上讲,RpfG 可以作为衔接蛋白发挥作用,以结合和抑制未磷酸化 WspR 的 DGC 活性,而与其 PDE 活性无关。WspR 的磷酸化削弱了其与 RpfG 的结合亲和力,并且还阻止了 RpfG 作为衔接蛋白的能力,这使得 Wsp 系统能够通过动态整合 DSF 系统以 c-di-GMP 依赖性方式调节生物膜形成。我们的研究结果证明了植物有益细菌和抗真菌细菌中 Wsp 和 DSF 系统之间以前未表征的串扰机制。

更新日期:2022-12-17
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