Cardiovascular Engineering and Technology ( IF 1.6 ) Pub Date : 2022-12-13 , DOI: 10.1007/s13239-022-00650-2
Connor Watson 1 , Lidya Abune 1 , Hicham Saaid 1 , Connie Wen 1 , Yong Wang 1 , Keefe B Manning 1, 2
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Introduction
Hydrogels offer a wide range of applications in the antithrombotic modification of biomedical devices. The functionalization of these hydrogels with potentially drug-laden nanoparticles in the context of deviceassociated turbulence is critically under-studied. Thus, the purpose of this study was to use a hydrogel-coating nitinol surface as a model to understand the functions of hydrogels and the capture of nanoparticles under clinically relevant flow conditions.
Methods
Nitinol was coated by an oligonucleotide (ON) functionalized hydrogel. Nanoparticles were functionalized with complementary oligonucleotides (CONs). The capture of CONfunctionalized nanoparticles by the ON-functionalized hydrogel surfaces was studied under both static and dynamic attachment conditions. Fluorescent-labelling of nanoparticles was utilized to assess capture efficacy and resistance to removal by device-relevant flow conditions.
Results
The specificity of the ON-CON bond was verified, exhibiting a dose-dependent attachment response. The hydrogel coating was resistant to stripping by flow, retaining >95% after exposure to one hour of turbulent flow. Attachment of nanoparticles to the hydrogel was higher in the static condition than under laminar flow (p < 0.01), but comparable to that of attachment under turbulent flow. Modified nitinol samples underwent one hour of flow treatment under both laminar and turbulent regimes and demonstrated decreased nanoparticle loss following static conjugation rather than turbulent conjugation (36.1% vs 53.8%, p < 0.05). There was no significant difference in nanoparticle functionalization by upstream injection between laminar and turbulent flow.
Conclusion
The results demonstrate promising potential of hydrogelfunctionalized nitinol for capturing nanoparticles using nucleic acid hybridization. The hydrogel structure and ONCON bond integrity both demonstrated a resistance to mechanical damage and loss of biomolecular functionalization by exposure to turbulence. Further investigation is warranted to highlight drug delivery and antithrombogenic modification applications of nanoparticle-functionalized hydrogels.
中文翻译:
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在血液接触装置的湍流条件下,水凝胶涂层镍钛诺与寡核苷酸修饰纳米粒子的性能
介绍
水凝胶在生物医学设备的抗血栓形成改造中提供了广泛的应用。在设备相关湍流的背景下,这些水凝胶与潜在载药纳米粒子的功能化研究严重不足。因此,本研究的目的是使用水凝胶涂层镍钛诺表面作为模型来了解水凝胶的功能和临床相关流动条件下纳米粒子的捕获。
方法
镍钛诺被寡核苷酸 (ON) 功能化水凝胶包裹。纳米粒子用互补寡核苷酸 (CON) 进行功能化。在静态和动态附着条件下研究了 ON 功能化水凝胶表面对 CON 功能化纳米粒子的捕获。纳米粒子的荧光标记被用来评估捕获效率和对设备相关流动条件去除的抵抗力。
结果
验证了 ON-CON 键的特异性,表现出剂量依赖性附着反应。水凝胶涂层可抵抗流动剥离,在湍流暴露一小时后保留 >95%。纳米粒子与水凝胶的附着在静态条件下比在层流下更高 (p < 0.01),但与在湍流下的附着相当。改性镍钛合金样品在层流和湍流状态下均经过一小时的流动处理,并证明静态共轭而非湍流共轭后纳米颗粒损失减少(36.1% 对 53.8%,p < 0.05)。层流和湍流之间的上游注入在纳米粒子功能化方面没有显着差异。
结论
结果表明,水凝胶功能化镍钛诺在使用核酸杂交捕获纳米粒子方面具有广阔的潜力。水凝胶结构和 ONCON 键完整性均表现出对机械损伤和暴露于湍流的生物分子功能丧失的抵抗力。需要进一步研究以突出纳米颗粒功能化水凝胶的药物输送和抗血栓形成修饰应用。