2,5-呋喃甲醛作为生物基交联剂替代戊二醛用于共价酶固定化,RSC Advances

当前位置： X-MOL 学术 › RSC Adv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

2,5-呋喃甲醛作为生物基交联剂替代戊二醛用于共价酶固定化
RSC Advances ( IF 3.9 ) Pub Date : 2022-12-14 , DOI: 10.1039/d2ra07153c
Chiara Danielli _{1,

2} , Luuk van Langen ₂ , Deborah Boes _{2,

3} , Fioretta Asaro ₁ , Serena Anselmi ₄ , Francesca Provenza _{4,

5} , Monia Renzi ₅ , Lucia Gardossi ₁

Affiliation

在寻求基于生物且更安全的戊二醛替代品时，我们研究了 2,5 二甲酰基呋喃 (DFF) 作为双功能交联剂，用于将葡糖淀粉酶共价固定在氨基官能化甲基丙烯酸树脂上。固定化实验和与戊二醛在四种不同浓度下的活化步骤的系统比较表明，DFF 在所有测试浓度下产生可比的酶活性。连续流动实验证实了用两种交联剂获得的固定化制剂具有相似的长期稳定性。与戊二醛相比，DFF 在水溶液中的 NMR 研究证明了一种更简单的行为，因为不能形成烯醇形式，并且仅观察到一水合物形式。与戊二醛不同的是，仅通过亚胺键形成。然而，在酸性 pH (4.5) 下也证实了共价固定的稳定性，这很可能是因为与芳香醛形成的亚胺键具有更高的稳定性。就毒性而言，DFF 具有难溶于水的优点，更重要的是，与表现出严重呼吸道毒性的戊二醛相比，挥发性差。我们使用海洋细菌费氏弧菌进行了初步的生态毒性测定，证明了两种二醛在测试浓度下的可比行为（低于毒性阈值）。

"点击查看英文标题和摘要"

2,5-Furandicarboxaldehyde as a bio-based crosslinking agent replacing glutaraldehyde for covalent enzyme immobilization

In the quest for a bio-based and safer substitute for glutaraldehyde, we have investigated 2,5 diformylfuran (DFF) as bifunctional crosslinking agent for the covalent immobilization of glucoamylase on amino-functionalized methacrylic resins. Immobilization experiments and systematic comparison with glutaraldehyde at four different concentrations for the activation step showed that DFF leads to comparable enzymatic activities at all tested concentrations. Continuous flow experiment confirms a similar long term stability of the immobilized formulations obtained with the two crosslinkers. The NMR study of DFF in aqueous solution evidenced a much simpler behaviour as compared to glutaraldehyde, since no enolic forms can form and only a mono-hydrated form was observed. Unlike in the case of glutaraldehyde, DFF reacts covalently with the primary amino groups via imine bond formation only. Nevertheless, the stability of the covalent immobilization was confirmed also at acidic pH (4.5), most probably because of the higher stability of the imine bonds formed with the aromatic aldehydes. In terms of toxicity DFF has the advantage of being poorly soluble in water and, more importantly, poorly volatile as compared to glutaraldehyde, which displays severe respiratory toxicity. We have performed preliminary ecotoxicity assays using Aliivibrio fischeri, a marine bacterium, evidencing comparable behaviour (below the toxicity threshold) for both dialdehydes at the tested concentrations.

更新日期：2022-12-14

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南