RAV1 和 BES1 表达的相互抑制调节拟南芥植物的生长发育,Journal of Integrative Plant Biology

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RAV1 和 BES1 表达的相互抑制调节拟南芥植物的生长发育
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2022-12-13 , DOI: 10.1111/jipb.13431
Dami Yang ₁ , Hyun-Young Shin ₁ , Hyun Kyung Kang ₁ , Yun Shang _{1,

2} , So Young Park ₃ , Dong-Hoon Jeong ₃ , Kyoung Hee Nam _{1,

2}

Affiliation

RAV1（与 ABI3/VP1 相关）是一种含有植物特异性 B3 和 AP2 结构域的转录因子，在许多植物物种中充当生长的负调节因子。RAV1的表达被油菜素类固醇 (BRs) 下调；大规模转录组分析表明，RAV1的表达先前被 BRI1-EMS-SUPPRESOR1 (BES1) 和 BRASSINAZOLE-RESISTANT1 (BZR1) 靶向，它们是 BR 信号传导过程的关键转录因子。使用RAV1过表达转基因植物，我们发现RAV1过表达降低了 BR 信号传导能力，导致 BR 生物合成基因和BES1的下调表达。此外，我们证明了 BES1，而不是 BZR1，直接与RAV1启动子结合并抑制RAV1表达，反之亦然；RAV1 也与BES1启动子结合并抑制BES1表达。这种相互抑制对 RAV1 和 BES1 具有特异性，因为 RAV1 表现出对BZR1启动子的结合活性但不抑制BZR1表达。我们观察到，在过表达RAV1的转基因bes1- D植物中，内源性BES1表达的抑制减弱了bes1-D中组成型激活的 BR 信号转导表型。的RNA测序分析RAV1过表达转基因植物和bes1-D突变植物揭示了由 RAV1 和 BES1 差异表达的基因以及由 RAV1 和 BES1 反向共同调节的基因。RAV1 和 BES1 调节不同的转录组，但共同调节负责生长和防御之间平衡的一组特定基因。这些结果表明RAV1和BES1的相互抑制转录活性为植物生长发育提供了良好的调控机制。

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Reciprocal inhibition of expression between RAV1 and BES1 modulates plant growth and development in Arabidopsis

RAV1 (Related to ABI3/VP1) is a plant-specific B3 and AP2 domain-containing transcription factor that acts as a negative regulator of growth in many plant species. The expression of RAV1 is downregulated by brassinosteroids (BRs); large-scale transcriptome analyses have shown that the expression of RAV1 was previously targeted by BRI1-EMS-SUPPRESOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1), which are critical transcription factors for the BR-signaling process. Using RAV1-overexpressing transgenic plants, we showed that RAV1 overexpression reduced the BR signaling capacity, resulting in the downregulation of BR biosynthetic genes and BES1 expression. Furthermore, we demonstrated that BES1, not BZR1, is directly bound to the RAV1 promoter and repressed RAV1 expression, and vice versa; RAV1 is also bound to the BES1 promoter and repressed BES1 expression. This mutual inhibition was specific to RAV1 and BES1 because RAV1 exhibited binding activity to the BZR1 promoter but did not repress BZR1 expression. We observed that constitutively activated BR signaling phenotypes in bes1-D were attenuated by the repression of endogenous BES1 expression in transgenic bes1-D plants overexpressing RAV1. RNA-sequencing analysis of RAV1-overexpressing transgenic plants and bes1-D mutant plants revealed differentially expressed genes by RAV1 and BES1 and genes that were oppositely co-regulated by RAV1 and BES1. RAV1 and BES1 regulated different transcriptomes but co-regulated a specific set of genes responsible for the balance between growth and defense. These results suggested that the mutual inhibitory transcriptional activities of RAV1 and BES1 provide fine regulatory mechanisms for plant growth and development.

更新日期：2022-12-13

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