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Intestine-on-a-chip for intestinal disease study and pharmacological research
VIEW ( IF 9.7 ) Pub Date : 2022-12-11 , DOI: 10.1002/viw.20220037 Dongxue Zhang 1 , Liang Qiao 1
VIEW ( IF 9.7 ) Pub Date : 2022-12-11 , DOI: 10.1002/viw.20220037 Dongxue Zhang 1 , Liang Qiao 1
Affiliation
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Intestinal diseases seriously affect the quality of life. The assays for intestinal disease study based on two-dimensional in vitro cell culture and animal models fail to model human physiology and pathophysiology accurately. As a bridge between in vitro cell culture and clinical pathological specimens, intestine-on-a-chip lined with living cells under dynamic flow can recapitulate the human intestinal microenvironment with high precision. Here, we summarize important elements for reconstructing the intestinal microenvironment and the mostly used intestine-on-a-chip models that simulate the basic intestinal microenvironment, and the intestinal microenvironment with pathogenic bacteria, probiotic bacteria, viruses, tumor cells, and parasites. We also introduce the commonly used analytical techniques, including immunofluorescence imaging, mass spectrometry, DNA/RNA-based analysis, and electrochemistry, which can be integrated with intestine chips for intestinal microenvironment characterization. We further review the application of intestine-on-a-chip for intestinal disease study and pharmacological research. Finally, we discuss the application prospects and challenges of intestine chips in biomedical and clinical research.
中文翻译：
肠道芯片用于肠道疾病研究和药理学研究
肠道疾病严重影响生活质量。基于二维体外细胞培养和动物模型的肠道疾病研究分析无法准确模拟人体生理学和病理生理学。作为体外细胞培养和临床病理标本之间的桥梁，在动态流动下排列有活细胞的片上肠可以高精度地再现人体肠道微环境。在这里，我们总结了重建肠道微环境的重要元素和最常用的模拟基本肠道微环境的片上肠道模型，以及具有致病菌、益生菌、病毒、肿瘤细胞和寄生虫的肠道微环境。我们还介绍了常用的分析技术，包括免疫荧光成像，质谱、基于 DNA/RNA 的分析和电化学，可与肠道芯片集成以表征肠道微环境。我们进一步回顾了肠道芯片在肠道疾病研究和药理学研究中的应用。最后，我们讨论了肠道芯片在生物医学和临床研究中的应用前景和挑战。
更新日期：2022-12-11
中文翻译：
肠道芯片用于肠道疾病研究和药理学研究
肠道疾病严重影响生活质量。基于二维体外细胞培养和动物模型的肠道疾病研究分析无法准确模拟人体生理学和病理生理学。作为体外细胞培养和临床病理标本之间的桥梁，在动态流动下排列有活细胞的片上肠可以高精度地再现人体肠道微环境。在这里，我们总结了重建肠道微环境的重要元素和最常用的模拟基本肠道微环境的片上肠道模型，以及具有致病菌、益生菌、病毒、肿瘤细胞和寄生虫的肠道微环境。我们还介绍了常用的分析技术，包括免疫荧光成像，质谱、基于 DNA/RNA 的分析和电化学，可与肠道芯片集成以表征肠道微环境。我们进一步回顾了肠道芯片在肠道疾病研究和药理学研究中的应用。最后，我们讨论了肠道芯片在生物医学和临床研究中的应用前景和挑战。
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