Fluorescence spectroscopy is gaining interest in the analysis and quantitative determination of different drugs. This study was carried out to investigate the fluorometric properties of the short-acting muscle relaxant mivacurium in its pure form, injection, and human plasma. It is nondepolarizing skeletal muscle relaxant for intravenous (IV) administration. Mivacurium shows a strong native fluorescence in methanol at 317 nm after excitation at 230 nm (Method I). The critical parameters that may influence the fluorescence of this drug were carefully studied. A linear response between concentration and fluorescence was constructed over the concentration range: 20.0 to 400.0 ng/mL with determination coefficient (r²) equal to 0.9998. Additionally, the correlation coefficient of the linear relationship (r) was found to be 0.9999 with a slope = 2.196 and intercept = −16.61. Limits of quantitation and detection were calculated mathematically to be 17.45 and 5.75 ng/mL respectively. Further estimation of mivacurium in spiked human plasma was performed by construction of specific calibration curve and the obtained correlation coefficient was 0.9948. Moreover, the ability to determine mivacurium in the presence of commonly co-administered drugs were investigated including propofol and thiopental. Method II includes the determination of MVC in the presence of propofol utilizing the first derivative synchronous fluorescence spectroscopy. The results of method II indicated acceptable percentage recoveries from 98.88 to 100.75 %. Statistical evaluation of the results revealed satisfactory accuracy and precision.

荧光光谱在不同药物的分析和定量测定中越来越受到关注。进行这项研究是为了研究短效肌肉松弛剂米库氯铵的纯形式、注射剂和人血浆的荧光特性。它是用于静脉内 (IV) 给药的非去极化骨骼肌松弛剂。在 230 nm 激发后，米库氯铵在 317 nm 的甲醇中显示出强烈的天然荧光（方法 I）。仔细研究了可能影响该药物荧光的关键参数。在以下浓度范围内构建浓度和荧光之间的线性响应：20.0 至 400.0 ng/mL，决定系数 (r ²) 等于 0.9998。此外，发现线性关系 (r) 的相关系数为 0.9999，斜率 = 2.196，截距 = −16.61。定量限和检测限经数学计算分别为 17.45 和 5.75 ng/mL。通过构建特定的校准曲线进一步评估加标人血浆中的米维库铵，获得的相关系数为 0.9948。此外，还研究了在异丙酚和硫喷妥钠等常用联合给药药物存在的情况下测定米库氯铵的能力。方法 II 包括使用一阶导数同步荧光光谱在异丙酚存在下测定 MVC。方法 II 的结果表明可接受的百分比回收率为 98.88 至 100.75%。