Journal of Electroanalytical Chemistry ( IF 4.1 ) Pub Date : 2022-12-12 , DOI: 10.1016/j.jelechem.2022.117070 Ming Lei , Yahui Zhang , Yongjie Hu , Yongping Dong , Wangbing Zhang
In this work, spinel-type CuGa2O4 nanoparticles were incorporated with MoS2 nanosheets to obtain uniform CuGa2O4/MoS2 nanocomposite which could be used to fabricate high efficient electrochemical platform based on its synergetic effect. Strong anodic electrogenerated chemiluminescence (ECL) of Ru(bpy)32+ obtained at MoS2 nanosheets modified electrode was greatly inhibited in the presence of CuGa2O4 nanoparticles. The resonance energy transfer (RET) between Ru(bpy)32+ ECL and CuGa2O4/MoS2 nanocomposite should be responsible for the decrease of ECL signal. Peroxide oxidase-like properties of CuGa2O4 nanoparticles endowed its ability to interact with cholesterol and the pathway of RET was cut off, leading to the recovery of ECL signal. Under the optimal condition, cholesterol could be sensitively detected in the range of 0.5–6 μM, and the limit of detection was determined as 0.1 μM. The modified electrode exhibited excellently repeatability, reproducibility, and long-term stability, and could be used to detect cholesterol in serum samples. The result suggested that the incorporation of spinel-type metal oxide with 2D material will bring new progress in ECL biosensor.
中文翻译:
CuGa2O4/MoS2纳米复合材料的合成及其电生化学发光传感应用
在这项工作中,尖晶石型CuGa 2 O 4纳米粒子与MoS 2纳米片结合得到均匀的CuGa 2 O 4 /MoS 2纳米复合材料,基于其协同效应可用于制造高效电化学平台。在CuGa 2 O 4纳米颗粒存在下,在MoS 2纳米片修饰电极上获得的Ru(bpy) 3 2+的强阳极电生化学发光(ECL) 受到极大抑制。Ru(bpy) 3 2+ ECL与CuGa 2 O 4之间的共振能量转移(RET)/MoS 2纳米复合材料应该是ECL信号减弱的原因。CuGa 2 O 4纳米粒子的过氧化物氧化酶样特性赋予其与胆固醇相互作用的能力,RET通路被切断,导致ECL信号恢复。在最佳条件下,胆固醇在0.5~6 μM范围内可被灵敏检测,检测限为0.1 μM。修饰电极表现出优异的重复性、再现性和长期稳定性,可用于检测血清样品中的胆固醇。结果表明,尖晶石型金属氧化物与二维材料的结合将为ECL生物传感器带来新的进展。