Fluorescence detection of multiple mRNAs has attracted great attention for disease diagnosis. In this work, a stimulus-responsive strategy for highly sensitive and accurate multiple mRNAs detection was proposed. This stimulus-responsive detection system was prepared by mesoporous silica nanoparticles (MSN), manganese dioxide (MnO₂) nanosheets, and DNA probes. DNA probes were loaded into the pores of MSN, which were closed with MnO₂ nanosheets. In the presence of glutathione (GSH) and target mRNAs, MnO₂ nanosheets were degraded by GSH, resulting in the release of DNA probes. These DNA probes hybridized to the corresponding target mRNA, thereby changing the fluorescence intensity of fluorophores of DNA probes, which could achieve the quantification of target mRNA. This system could simultaneously detect survivin mRNA and Thymidine kinase 1 mRNA at low background levels with relative limits of detection of 0.9 nM and 0.7 nM, respectively. Moreover, this assay has been successfully applied to detect multiple mRNAs with adequate anti-interference ability in the biological sample.

多种mRNA的荧光检测在疾病诊断中引起了极大的关注。在这项工作中，提出了一种用于高灵敏度和准确检测多个 mRNA 的刺激响应策略。该刺激响应检测系统由介孔二氧化硅纳米粒子 (MSN)、二氧化锰 (MnO ₂ ) 纳米片和 DNA 探针制备而成。DNA 探针被加载到 MSN 的孔隙中，这些孔隙被 MnO ₂纳米片封闭。在存在谷胱甘肽 (GSH) 和目标 mRNA 的情况下，MnO ₂纳米片被 GSH 降解，导致 DNA 探针的释放。这些DNA探针与相应的靶mRNA杂交，从而改变DNA探针荧光团的荧光强度，从而实现对靶mRNA的定量。该系统可以在低背景水平下同时检测存活蛋白 mRNA 和胸苷激酶 1 mRNA，相对检测限分别为 0.9 nM 和 0.7 nM。此外，该测定已成功应用于检测生物样品中具有足够抗干扰能力的多种 mRNA。