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Magnetic Large-Mesoporous Nanoreactors Enable Enzymatically Regulated Background-Free and Persistently Signalling Diseases Diagnosis
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2022-12-09 , DOI: 10.1002/adfm.202212317 Juan Li 1 , Jinhua Huang 2 , Yongjian Jiang 3 , Limin Wu 4 , Yonghui Deng 1, 4
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2022-12-09 , DOI: 10.1002/adfm.202212317 Juan Li 1 , Jinhua Huang 2 , Yongjian Jiang 3 , Limin Wu 4 , Yonghui Deng 1, 4
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Diverse diseases and increasing prevalence pose a serious threat to public health. Point-of-care testing (POCT) techniques have imposed superior requirements over sensitivity, selectivity, robustness, affordability, and high-throughput. However, transient signal, complex sample pretreatment, and low signal-to-noise ratio make POCT severely limited in detection accuracy, efficiency, and sensitivity. Here, an enzyme-assisted magnetic large-mesoporous nanoreactor (FS) is constructed for achieving persistent-chemiluminescence signal output and eliminating matrix interference in disease diagnosis. The core-shell structured FS synthesized via an interface coassembly method exhibits uniform size, large and open mesopores (≈22 nm), and intrinsic magnetic separability. Such unique FS acts as efficient nanoreactor for confined cascade reactions enable efficient persistent-chemiluminescence (pCL) signal transduction and high-SNR chromogenic analysis of diverse biomarkers. The developed pCL assays facilitate high-sensitive determination of chronic disease biomarkers glucose and uric acid with detection limits (DL) of 5.4 mg L−1 and 151.2 ng L−1, respectively. The proposed chromogenic immunoassay enables an ultrasensitive and visual determination of alpha-fetoprotein with a DL of 1.2 ng L−1, which is superior to previously published immunoassays. The feasibility of the developed methods for real-world applications are demonstrated in 159 clinical serum samples, and the determination results agree well with the clinical data. The proposed technique is expected to promote highly sensitive disease diagnosis in primary medical institutions and resource-limited areas since not relying on expensive automatic sampling and testing instruments. The good flexibility of the customizable nanoreactor makes it a powerful tool for developing various POCT techniques for rapid, sensitive, and accurate diseases diagnosis.
中文翻译：
磁性大介孔纳米反应器使酶调节的无背景和持续信号疾病诊断成为可能
多种疾病和日益流行的疾病对公众健康构成严重威胁。床旁检测 (POCT) 技术对灵敏度、选择性、稳健性、可负担性和高通量提出了更高的要求。然而瞬态信号、复杂的样本预处理和低信噪比等因素使得POCT在检测精度、效率和灵敏度方面受到严重限制。在这里，构建了一种酶辅助磁性大介孔纳米反应器 (FS)，以实现持久化学发光信号输出并消除疾病诊断中的基质干扰。通过界面组装法合成的核壳结构 FS 具有均匀的尺寸、大而开放的中孔 (≈22 nm) 和固有的磁可分离性。这种独特的 FS 充当用于受限级联反应的高效纳米反应器，可实现高效的持久化学发光 (pCL) 信号转导和多种生物标志物的高 SNR 显色分析。开发的 pCL 检测有助于高灵敏度地测定慢性病生物标志物葡萄糖和尿酸，检测限 (DL) 为 5.4 mg·L分别为-1和 151.2 ng L -1。拟议的显色免疫测定法能够以超灵敏和视觉方式测定甲胎蛋白，DL 为 1.2 ng L -1，优于以前发表的免疫测定。在 159 份临床血清样本中证明了所开发方法在实际应用中的可行性，测定结果与临床数据吻合良好。由于不依赖昂贵的自动采样和检测仪器，该技术有望在基层医疗机构和资源有限地区促进高灵敏度疾病诊断。可定制纳米反应器的良好灵活性使其成为开发用于快速、灵敏和准确疾病诊断的各种 POCT 技术的有力工具。
更新日期：2022-12-09
中文翻译：
磁性大介孔纳米反应器使酶调节的无背景和持续信号疾病诊断成为可能
多种疾病和日益流行的疾病对公众健康构成严重威胁。床旁检测 (POCT) 技术对灵敏度、选择性、稳健性、可负担性和高通量提出了更高的要求。然而瞬态信号、复杂的样本预处理和低信噪比等因素使得POCT在检测精度、效率和灵敏度方面受到严重限制。在这里，构建了一种酶辅助磁性大介孔纳米反应器 (FS)，以实现持久化学发光信号输出并消除疾病诊断中的基质干扰。通过界面组装法合成的核壳结构 FS 具有均匀的尺寸、大而开放的中孔 (≈22 nm) 和固有的磁可分离性。这种独特的 FS 充当用于受限级联反应的高效纳米反应器，可实现高效的持久化学发光 (pCL) 信号转导和多种生物标志物的高 SNR 显色分析。开发的 pCL 检测有助于高灵敏度地测定慢性病生物标志物葡萄糖和尿酸，检测限 (DL) 为 5.4 mg·L分别为-1和 151.2 ng L -1。拟议的显色免疫测定法能够以超灵敏和视觉方式测定甲胎蛋白，DL 为 1.2 ng L -1，优于以前发表的免疫测定。在 159 份临床血清样本中证明了所开发方法在实际应用中的可行性，测定结果与临床数据吻合良好。由于不依赖昂贵的自动采样和检测仪器，该技术有望在基层医疗机构和资源有限地区促进高灵敏度疾病诊断。可定制纳米反应器的良好灵活性使其成为开发用于快速、灵敏和准确疾病诊断的各种 POCT 技术的有力工具。
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