用于检测单个纳米颗粒和小分子的双纳米移液器,Analytical Chemistry

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用于检测单个纳米颗粒和小分子的双纳米移液器
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-12-10 , DOI: 10.1021/acs.analchem.2c03344
Xinhe Zheng ₁ , Junjie Liu ₁ , Mingzhi Li ₁ , Yutong Hua ₁ , Xu Liang ₁ , Shudong Zhang ₁ , Xianhao Zhang ₁ , Yuanhua Shao ₁

Affiliation

纳米孔传感由于其免标记和高灵敏度的特点而蓬勃发展。作为一种新型纳米孔，液滴在双纳米移液器的孔口处形成，允许分析物以相对较低的速度通过两个通道转移，并提高信噪比。然而，基于电流阻塞原理的纳米孔传感要求孔径大小与单个实体相当，这对小分子的直接检测提出了巨大挑战。在这项工作中，成功检测到用巯基聚乙二醇 (PEG-SH) 或适体修饰的金纳米粒子 (Au NPs)。可以灵敏地区分 Au NP 的尺寸差异以及 Au NP 与双纳米移液器之间的相互作用。此外，用设计的适配体修饰的Au NPs在捕获相应的小分子（如多巴胺和血清素）后会产生不同的阻断电流。即使是非电活性离子，如钾离子，也可以被检测到，这是基于氧化还原反应难以检测到的，进一步说明了纳米粒子表面性质的变化是检测的原因。该工作拓展了纳米移液器对金纳米粒子传感的应用，为小分子检测提供了一个通用平台，在生物传感领域具有潜在的应用前景。并进一步说明纳米粒子表面性质的变化是检测的原因。该工作拓展了纳米移液器对金纳米粒子传感的应用，为小分子检测提供了一个通用平台，在生物传感领域具有潜在的应用前景。并进一步说明纳米粒子表面性质的变化是检测的原因。该工作拓展了纳米移液器对金纳米粒子传感的应用，为小分子检测提供了一个通用平台，在生物传感领域具有潜在的应用前景。

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Dual-Nanopipettes for the Detection of Single Nanoparticles and Small Molecules

Nanopore sensing is blooming due to its label-free and high sensitivity features. As a novel nanopore, a droplet is formed at the orifice of a dual-nanopipette, which allows for the translocation of analytes through the two channels at a relatively low speed and the promotion of signal-to-noise ratio. However, nanopore sensing based on the principle of current blockage requires the pore size to be comparable to that of the single entity, which poses a huge challenge for the direct detection of small molecules. In this work, gold nanoparticles (Au NPs) modified with sulfhydryl poly(ethylene glycol) (PEG-SH) or aptamers were detected successfully. The size difference of Au NPs and the interaction between Au NPs and dual-nanopipettes could be distinguished sensitively. Furthermore, Au NPs modified with designed aptamers will produce different blocking current after capturing the corresponding small molecules (e.g., dopamine and serotonin). Even non-electroactive ions, such as potassium ions, can also be detected, which is difficult to sense based on redox reactions, and further illustrates that the change of surface properties of nanoparticles is responsible for the detection. This work expands the application of nanopipette sensing for Au NPs and provides a universal platform for the small-molecule detection, which has the potential application in biosensing.

更新日期：2022-12-10

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