Tebufenpyrad is classified as a pyrazole acaricide and insecticide. It is widely used for several crops, especially in greenhouses, in several countries. While its unfavorable effects on non-target organisms have already been established, relatively little is known about its reproductive toxicity. Therefore, we demonstrated the biochemical effects of tebufenpyrad using porcine trophectoderm and porcine luminal epithelial cells, which are involved in implantation. We found that tebufenpyrad had antiproliferative effects and reduced cell viability. Tebufenpyrad also triggered apoptosis and excessive reactive oxygen species production. Furthermore, it induced cell cycle arrest in the G1 phase and disrupted calcium homeostasis in the cytosol and mitochondria. MAPK signaling pathways and the crosstalk among them were altered following tebufenpyrad treatment. In addition, the migration ability of cells was reduced after treatment with tebufenpyrad. Lastly, tebufenpyrad influenced the expression of genes related to pregnancy. Collectively, these results reveal the mechanism of the biochemical and physiological effects of tebufenpyrad to both trophectoderm and uterine cells and suggest that tebufenpyrad reduces the potential of successful implantation.

Tebufenpyrad 被归类为吡唑类杀螨剂和杀虫剂。它在多个国家被广泛用于多种作物，尤其是温室中。虽然它对非靶标生物的不利影响已经确定，但对其生殖毒性知之甚少。因此，我们使用参与植入的猪滋养外胚层和猪管腔上皮细胞证明了 tebufenpyrad 的生化作用。我们发现 tebufenpyrad 具有抗增殖作用并降低细胞活力。Tebufenpyrad 还引发细胞凋亡和过量活性氧的产生。此外，它在 G1 期诱导细胞周期停滞并破坏细胞质和线粒体中的钙稳态。MAPK 信号通路和它们之间的串扰在 tebufenpyrad 处理后发生了改变。此外，tebufenpyrad处理后细胞的迁移能力降低。最后，tebufenpyrad 影响了与妊娠有关的基因的表达。总的来说，这些结果揭示了吡虫啉对滋养外胚层和子宫细胞的生化和生理作用的机制，并表明吡虫啉降低了成功着床的可能性。