电针对IBS-D大鼠内脏敏感性及结肠NGF、TrkA、TRPV1表达的影响,Zhongguo zhen jiu = Chinese acupuncture & moxibustion

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电针对IBS-D大鼠内脏敏感性及结肠NGF、TrkA、TRPV1表达的影响
Zhongguo zhen jiu = Chinese acupuncture & moxibustion Pub Date : 2022-12-12 , DOI: 10.13703/j.0255-2930.20220130-0005
Yi-Chen Yang ₁ , Zi-Xian Zhou ₁ , Ting Xue ₂ , Yuan-Hui Feng ₁ , Jun-Tao Chen ₁ , Tu-Nan Wang ₃ , Jia-Yu Zhao ₄ , Liu-Jing Wang ₅ , Peng Zhang ₆ , Li-Ping Zhang ₇ , Hui-Fang Ma ₁

Affiliation

目的观察电针“天枢”(ST 25)和“上巨虚”(ST 37)对精神状态、内脏敏感性及神经生长因子(NGF)、酪氨酸激酶受体A(TrkA)蛋白表达的影响。腹泻型肠易激综合征（IBS-D）大鼠结肠组织瞬时受体电位香草素1（TRPV1）和瞬时受体电位香草素1（TRPV1），探讨其治疗IBS-D的可能机制。方法SPF级雄性SD大鼠36只，随机分为空白组、模型组、电针组和西药组，每组9只。模型组、电针组、西药组采用二硝基苯磺酸（DNBS）灌肠联合慢性束缚应激法建立IBS-D模型。EA组，EA在《天枢》上应用 (ST 25)和“上聚序”(ST 37)，散密波，频率2Hz/100Hz，每次20分钟，每天一次，连续7天。西药组给予匹维溴铵混悬液灌胃(15 mg•kg-1•d-1)，连续7 d。模型建立前后及干预后观察体重、24 h摄食量和粪便含水量，通过腹部退缩反射（AWR）检测内脏敏感性；干预后,采用高架十字迷宫(EPM)试验评价4组患者的精神状态,免疫组化及Western blot检测4组NGF、TrkA、TRPV1蛋白表达。结果造模后，与空白组比较，体质量和24 h摄食量均降低（P<0.05），模型组、电针组和西药组AWR第一收缩潜伏期缩短，AWR收缩波数增加（P<0.05），粪便含水量增加（P<0.05）。干预后，与空白组比较，模型组EPM开臂停留时间比（OT%）降低（P<0.05），结肠组织NGF、TrkA、TRPV1蛋白表达升高（P<0.05） ; 与模型组比较，体质量和24 h摄食量增加（P<0.05），AWR第一收缩期潜伏期延长，AWR收缩波数减少（P<0.05），粪便含水量降低(P<0.05)，结肠组织EPM的OT%升高(P<0.05)，NGF、TrkA、TRPV1蛋白表达降低(P<0.05)。05)在电针组和西药组。结论电针“天枢”(ST 25)、“上巨虚”(ST 37)可减轻IBS-D大鼠的焦虑、抑郁样行为，下调结肠组织NGF、TrkA、TRPV1蛋白表达，从而改善IBS-D大鼠结肠组织中NGF、TrkA、TRPV1蛋白的表达，从而改善IBS-D大鼠的焦虑、抑郁样行为。从而降低内脏敏感度，缓解症状。

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[Effect of electroacupuncture on visceral sensitivity and colonic NGF, TrkA, TRPV1 expression in IBS-D rats].

OBJECTIVE To observe the effect of electroacupuncture (EA) at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) on mental state, visceral sensitivity and protein expression of nerve growth factor (NGF), tyrosine kinase receptor A (TrkA) and transient receptor potential vanilloid 1 (TRPV1) of colonic tissue in diarrhea-predominant irritable bowel syndrome (IBS-D) rats, and to explore its possible mechanism on treating IBS-D. METHODS A total of 36 male SD rats of SPF grade were randomized into a blank group, a model group, an EA group and a western medication group, 9 rats in each group. In the model group, the EA group and the western medication group, IBS-D model was established by enema of dinitrobenzene sulfonic acid (DNBS) combined with chronic restraint stress method. In the EA group, EA was applied at "Tianshu" (ST 25) and "Shangjuxu" (ST 37), with disperse-dense wave, in frequency of 2 Hz/100 Hz, 20 min each time, once a day for 7 days. In the western medication group, pinaverium bromide suspension was given by gavage (15 mg•kg-1•d-1) for 7 days. Before and after model establishment, and after intervention, the body mass, 24 h food intake and fecal water content were observed, the visceral sensitivity was detected by abdominal withdrawal reflex (AWR); after intervention, the mental state was evaluated by elevated plus maze (EPM) test, the protein expression of NGF, TrkA and TRPV1 was detected by immunohistochemistry and Western blot in the 4 groups. RESULTS After model establishment, compared with the blank group, the body mass and 24 h food intake were decreased (P<0.05), first systolic latency of AWR was shortened and number of contraction wave of AWR was increased (P<0.05), and fecal water content was increased (P<0.05) in the model group, the EA group and the western medication group. After intervention, compared with the blank group, open arm residence time ratio (OT%) of EPM was decreased (P<0.05) and protein expression of NGF, TrkA, TRPV1 in colonic tissue was increased in the model group (P<0.05); compared with the model group, the body mass and 24 h food intake were increased (P<0.05), first systolic latency of AWR was lengthened and number of contraction wave of AWR was decreased (P<0.05), the fecal water content was decreased (P<0.05), OT% of EPM was increased (P<0.05), and protein expression of NGF, TrkA, TRPV1 in colonic tissue was decreased (P<0.05) in the EA group and the western medication group. CONCLUSION Electroacupuncture at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) can relieve the anxiety and depression-like behaviors in IBS-D rats, down-regulate the protein expression of NGF, TrkA, TRPV1 in colonic tissue, so as to reduce the visceral sensitivity and relieve symptoms.

更新日期：2022-12-10

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