A highly sensitive and convenient amplified luminescent proximity homogeneous assay (AlphaLISA) method with high throughput and automation potential was developed for quantitation of serum Gastrin-17 (G-17) levels, which can facilitate the early diagnosis of atrophic gastritis in people at high risk of gastric cancer using a non-invasive approach. In this study, donor and acceptor beads with modified carboxyl groups on the surface were directly coupled to anti-G-17 antibodies through activation was proposed for application in the development of the new AlphaLISA, which can effectively simplify the steps and shorten the reaction time to achieve faster detection. Therefore, the G-17-AlphaLISA only needs to react for 15 min to obtain good analysis results. The proposed method has a wider detection range than commercial enzyme-linked immunosorbent assay (ELISA) kits (0.12–112.8 pmol/L > 0.5–40 pmol/L). In addition, results of G-17-AlphaLISA and ELISA had good correlation and agreement (ρ = 0.936). Importantly, the developed method may be more suitable for the large-scale screening of people at high risk for gastric cancer than traditional ELISA and provides a novel solution for other biomarkers that require accurate, highly sensitive, and high throughput detection.

开发了一种具有高通量和自动化潜力的高度灵敏、方便的放大发光邻近均相测定 (AlphaLISA) 方法，用于定量血清胃泌素-17 (G-17) 水平，有助于早期诊断高危人群的萎缩性胃炎使用非侵入性方法治疗胃癌。本研究提出将表面修饰羧基的供体珠和受体珠通过活化直接偶联抗G-17抗体，应用于新型AlphaLISA的开发，可有效简化步骤，缩短反应时间以实现更快的检测。因此，G-17-AlphaLISA 只需反应 15 分钟即可获得良好的分析结果。所提出的方法比商业酶联免疫吸附测定 (ELISA) 试剂盒具有更广泛的检测范围 (0.12–112.8 pmol/L > 0.5–40 pmol/L)。此外，G-17-AlphaLISA和ELISA的结果具有良好的相关性和一致性（ρ  = 0.936）。重要的是，所开发的方法可能比传统的 ELISA 更适用于胃癌高危人群的大规模筛查，并为其他需要准确、高灵敏度和高通量检测的生物标志物提供了一种新的解决方案。