SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones,ACS Applied Materials & Interfaces

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SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2022-12-08 , DOI: 10.1021/acsami.2c18053
Rongke Gao _{1,

2} , Yuanshuo Mao _{1,

2} , Chao Ma ₁ , Yeru Wang ₁ , Huakun Jia ₁ , Xiaozhe Chen ₁ , Yang Lu ₁ , Dongzhi Zhang ₁ , Liandong Yu ₁

Affiliation

We developed a new plasmonic nanostripe microcone array (PNMA) substrate-integrated microfluidic chip for the simultaneous surface-enhanced Raman scattering (SERS)-based immunoassay of the creatine kinase MB isoenzyme (CK-MB) and cardiac troponin (cTnI) cardiac markers. The conventional immunoassay usually employs a microtiter plate as the solid capture plate to form the immunocomplexes. However, the two-dimensional (2D) surface of the microtiter plate limits the capture efficiency of the target antigens due to the steric hindrance effect. To address this issue, a gold film-coated microcone array with nanostripes was developed that can provide a large surface area for capture antibody conjugation and serve as a SERS-active substrate. This unique nano–microhierarchical structure showed an excellent light trapping effect and induced surface plasmon resonance to further enhance the Raman signals of the SERS nanoprobes. It significantly improved the sensitivity and applicability of SERS-based immunoassay on the microfluidic chip. With this integrated microfluidic chip, we successfully performed the simultaneous detection of CK-MB and cTnI, and the detection limit can reach 0.01 ng mL^–1. It is believed that the PNMA substrate-integrated microfluidic chip would play a critical role in the rapid and sensitive diagnostics of cardiac diseases.

中文翻译：

基于 SERS 的心肌梗死生物标志物免疫测定在具有等离子纳米条纹微锥的微流控芯片上

我们开发了一种新的等离子体纳米条纹微锥阵列 (PNMA) 基板集成微流控芯片，用于同时进行基于表面增强拉曼散射 (SERS) 的肌酸激酶 MB 同工酶 (CK-MB) 和心肌肌钙蛋白 (cTnI) 心脏标记物的免疫测定。传统的免疫测定通常采用微量滴定板作为固体捕获板来形成免疫复合物。然而，由于空间位阻效应，微量滴定板的二维 (2D) 表面限制了目标抗原的捕获效率。为了解决这个问题，开发了一种带有纳米条纹的金膜涂层微锥阵列，它可以为捕获抗体偶联提供大的表面积，并作为 SERS 活性底物。这种独特的纳米-微观层次结构显示出优异的光捕获效应和诱导表面等离子体共振，进一步增强了 SERS 纳米探针的拉曼信号。它显着提高了微流控芯片上基于 SERS 的免疫分析的灵敏度和适用性。利用这种集成微流控芯片，我们成功地进行了CK-MB和cTnI的同时检测，检测限可达0.01 ng·mL^–1。相信PNMA基板集成微流控芯片将在心脏疾病的快速灵敏诊断中发挥关键作用。

更新日期：2022-12-08

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