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Bulk RNA sequencing analysis of developing human induced pluripotent cell-derived retinal organoids
Scientific Data ( IF 5.8 ) Pub Date : 2022-12-09 , DOI: 10.1038/s41597-022-01853-x
Devansh Agarwal 1, 2 , Rian Kuhns 3 , Christos N Dimitriou 3 , Emmalyn Barlow 3 , Karl J Wahlin 1 , Ray A Enke 3, 4
Affiliation  

Retinogenesis involves the transformation of the anterior developing brain into organized retinal lamellae coordinated by intricate gene signalling networks. This complex process has been investigated in several model organisms such as birds, fish, mammals and amphibians, yet many facets of retinal development are different in humans and remain unexplored. In this regard, human pluripotent stem cell (hPSC)-derived 3D retinal organoids and Next Generation Sequencing (NGS) have emerged as key technologies that have facilitated the discovery of previously unknown details about cell fate specification and gene regulation in the retina. Here we utilized hPSCs integrated with fluorescent reporter genes (SIX6-p2A-eGFP/CRX-p2A-h2b-mRuby3) to generate retinal organoids and carry out bulk RNA sequencing of samples encompassing the majority of retinogenesis (D0-D280). This data set will serve as a valuable reference for the vision research community to characterize differentially expressed genes in the developing human eye.



中文翻译:


开发人诱导多能细胞源性视网膜类器官的批量 RNA 测序分析



视网膜发生涉及将前部发育中的大脑转变为由复杂的基因信号网络协调的有组织的视网膜薄片。这一复杂的过程已经在鸟类、鱼类、哺乳动物和两栖动物等多种模式生物中进行了研究,但人类视网膜发育的许多方面与人类不同,仍未得到探索。在这方面,人类多能干细胞 (hPSC) 衍生的 3D 视网膜类器官和下一代测序 (NGS) 已成为关键技术,有助于发现先前未知的有关视网膜细胞命运规范和基因调控的细节。在这里,我们利用与荧光报告基因 (SIX6-p2A-eGFP/CRX-p2A-h2b-mRuby3) 集成的 hPSC 来生成视网膜类器官,并对涵盖大部分视网膜发生 (D0-D280) 的样品进行批量 RNA 测序。该数据集将为视觉研究界提供有价值的参考,以表征发育中的人眼中的差异表达基因。

更新日期:2022-12-09
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