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Molecular characterization of a new geminivirus isolated from tomato with curly top symptoms and development of its infectious clone
Journal of General Plant Pathology ( IF 1.0 ) Pub Date : 2022-12-09 , DOI: 10.1007/s10327-022-01112-2
Kenji Kubota , Yasuhiro Tomitaka , Tomio Usugi , Hiroyuki Hamada , Hiroki Ito , Atsushi Kuwana , Shinya Tsuda

A new geminivirus was isolated from field-grown tomatoes with leaf yellowing and curly top symptoms in Fukushima, Japan in 2009–2010. Sequence comparisons of the full-length genome of the virus and other geminiviruses revealed its close relationship to common bean curly stunt virus (CBCSV), with 86.1% nucleotide sequence identity. The virus encodes six open reading frames (ORFs) that had a very high sequence identity (84.3–92.6%) with those of CBCSV, but it lacks ORF V3. The agroinoculation of the cloned genome induced stunting on the tobacco plants, and the graft inoculation of tobacco scions onto tomato rootstock reproduced curly top symptoms. The name "tomato curly top virus" is proposed for this virus.



中文翻译:

从具有卷曲顶部症状的番茄中分离出的一种新双生病毒的分子特征及其感染性克隆的开发

2009-2010 年在日本福岛,从田间种植的具有叶片黄化和卷曲顶部症状的西红柿中分离出一种新的双生病毒。该病毒和其他双生病毒的全长基因组序列比较揭示了它与菜豆卷曲矮化病毒 (CBCSV) 的密切关系,具有 86.1% 的核苷酸序列同一性。该病毒编码六个开放阅读框 (ORF),与 CBCSV 具有非常高的序列同一性 (84.3–92.6%),但它缺少 ORF V3。克隆基因组的农用接种诱导了烟草植株发育迟缓,将烟草接穗嫁接接种到番茄砧木上再现了卷曲顶部症状。为该病毒提议命名为“番茄卷顶病毒”。

更新日期:2022-12-10
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