Miao sour soup influences serum lipid via regulation of high-fat diet-induced intestinal flora in obese rats,Food Science & Nutrition

当前位置： X-MOL 学术 › Food Sci. Nutr. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Miao sour soup influences serum lipid via regulation of high-fat diet-induced intestinal flora in obese rats
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2022-12-07 , DOI: 10.1002/fsn3.3136
Qianqian Zhou _{1,

2} , Zihan Qu ₁ , Nanlan Wang ₁ , Huijuan Liu ₁ , Hongmei Yang ₁ , Huiqun Wang _{1,

3}

Affiliation

Obesity is associated with the gut microbiota and has been shown to cause gut microbiota disturbances. Our previous studies have demonstrated that Miao sour soup (SS) contains abundant short-chain fatty acids (SCFAs) which can be used as energy substrates of intestinal flora to selectively stimulate their growth and reproduction. Therefore, we explored whether the intestinal microbiota of rats with high-fat diet-induced obesity could be restored to normal by SS intervention. Male obese rats were divided into five groups randomly after successful modeling of obese rats: normal diet, high-fat diet (HDF), HFD + SS, HFD with antibiotic, and HFD with antibiotic + SS. After 12 weeks of intervention, the weight and serum lipid of obese rats decreased. Furthermore, 16S rRNA analysis showed an imbalance and a decrease in the abundance and diversity of intestinal flora in obese rats, which improved after SS intervention. At the phylum level, Firmicutes increased while Proteobacteria decreased. The composition of the intestinal flora recovered at the genus level, inhibiting the reproduction of pathogenic bacteria, while the levels of SCFA-producing bacteria such as Blautia and Lactococcus and the levels of SCFAs in cecal contents increased. In addition, SS reduced the levels of TNF-α and IL-6 in the intestinal mucosa of obese rats, increased the contents of PYY and GLP-1 in colon tissue, and increased the expression of tight junction protein Occludin and ZO-1 in the intestinal epithelium. Taken together, SS can regulate the intestinal flora of obese rats and improve the intestinal flora to facilitate weight loss and lipid reduction.

中文翻译：

苗酸汤通过调节肥胖大鼠肠道菌群影响血脂

肥胖与肠道微生物群有关，并已被证明会导致肠道微生物群紊乱。我们前期的研究表明，苗酸汤（SS）中含有丰富的短链脂肪酸（SCFA），可以作为肠道菌群的能量底物，选择性地刺激其生长和繁殖。因此，我们探讨了SS干预能否使高脂饮食肥胖大鼠的肠道菌群恢复正常。雄性肥胖大鼠造模成功后随机分为5组：正常饮食组、高脂饮食组（HDF）、HFD+SS、HFD+抗生素组、HFD+抗生素+SS组。干预12周后，肥胖大鼠体重和血脂均下降。此外，16S rRNA分析显示，肥胖大鼠肠道菌群的丰度和多样性不平衡、减少，但在SS干预后有所改善。在门水平上，厚壁菌门增加，变形菌门减少。肠道菌群组成在属水平上恢复，病原菌繁殖受到抑制，布劳氏菌属、乳球菌属等产短链脂肪酸细菌的水平以及盲肠内容物中短链脂肪酸的水平上升。此外，SS还降低了肥胖大鼠肠粘膜中TNF-α和IL-6的水平，增加了结肠组织中PYY和GLP-1的含量，并增加了肥胖大鼠中紧密连接蛋白Occludin和ZO-1的表达。肠上皮。综合来看，SS可以调节肥胖大鼠的肠道菌群，改善肠道菌群，有利于减肥降脂。

更新日期：2022-12-07

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南