Inactivation of bioaerosols is critical to prevent the spread of microbial pathogens in air. Photocatalytic inactivation processes are environmental friendliness, while time-consuming and insufficient reactive oxygen species (ROSs) generation to irreversibly inactivate bacteria in bioaerosols. In this work, a novel photocatalytic ozonation (PCO) system was constructed, consisting of in situ grown NiFeOOH nanosheets on nickel foam (NiFeOOH NSs/NF) and trace concentration of ozone (O₃). Specifically, the PCO system exhibited remarkable photocatalytic inactivation efficiency (99.99%) within an ultrashort retention time of 8.07 s (RH = 90%) while no bacteria survived after 24-h cultivation. The irreversible inactivation of bacteria in this PCO system attributes to the synergistic effect of physical destruction by NiFeOOH NSs/NF and oxidative damage by photogenerated ROSs. This research provides a better alternative for efficient inactivation of bioaerosols and opens the door for the application of PCO system in reducing the exposure risk of bioaerosols.

生物气溶胶的灭活对于防止微生物病原体在空气中传播至关重要。光催化灭活过程对环境友好，但耗时且活性氧 (ROS) 的产生不足以不可逆地灭活生物气溶胶中的细菌。在这项工作中，构建了一种新型光催化臭氧化 (PCO) 系统，该系统由在泡沫镍上原位生长的 NiFeOOH 纳米片 (NiFeOOH NSs/NF) 和痕量浓度的臭氧 (O ₃). 具体而言，PCO 系统在 8.07 秒（RH = 90%）的超短保留时间内表现出显着的光催化灭活效率（99.99%），而培养 24 小时后没有细菌存活。该 PCO 系统中细菌的不可逆失活归因于 NiFeOOH NSs/NF 的物理破坏和光生 ROS 的氧化损伤的协同作用。该研究为生物气溶胶的高效灭活提供了更好的选择，并为PCO系统在降低生物气溶胶暴露风险方面的应用打开了大门。