Fast and Dynamic Mapping of the Protein Corona on Nanoparticle Surfaces by Photocatalytic Proximity Labeling,Advanced Materials

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Fast and Dynamic Mapping of the Protein Corona on Nanoparticle Surfaces by Photocatalytic Proximity Labeling
Advanced Materials ( IF 27.4 ) Pub Date : 2022-12-07 , DOI: 10.1002/adma.202206636
Zibin Zhang ₁ , Junji Ren ₁ , Wenbing Dai ₁ , Hua Zhang ₁ , Xueqing Wang ₁ , Bing He ₁ , Qiang Zhang ₁

Affiliation

Protein corona broadly affects the delivery of nanomedicines in vivo. Although it has been widely studied by multiple strategies like centrifugal sedimentation, the rapidly forming mechanism and the dynamic structure of the protein corona at the seconds level remains challenging. Here, a photocatalytic proximity labeling technology in nanoparticles (nano-PPL) is developed. By fabricating a “core-shell” nanoparticle co-loaded with chlorin e6 catalyst and biotin-phenol probe, nano-PPL technology is validated for the rapid and precise labeling of corona proteins in situ. Nano-PPL significantly improves the temporal resolution of nano-protein interactions to 5 s duration compared with the classical centrifugation method (>30 s duration). Furthermore, nano-PPL achieves the fast and dynamic mapping of the protein corona on anionic and cationic nanoparticles, respectively. Finally, nano-PPL is deployed to verify the effect of the rapidly formed protein corona on the initial interaction of nanoparticles with cells. These findings highlight a significant methodological advance toward nano-protein interactions in the delivery of nanomedicines in vivo.

中文翻译：

通过光催化邻近标记快速动态地映射纳米颗粒表面的蛋白质电晕

蛋白质电晕广泛影响纳米药物在体内的递送。尽管已经通过离心沉降等多种策略对其进行了广泛研究，但秒级蛋白质电晕的快速形成机制和动态结构仍然具有挑战性。在这里，开发了纳米粒子 (nano-PPL) 中的光催化邻近标记技术。通过制造与二氢卟酚 e6 催化剂和生物素-苯酚探针共同负载的“核-壳”纳米粒子，纳米 PPL 技术被验证用于原位快速和精确标记电晕蛋白。与经典离心方法（>30 秒持续时间）相比，纳米 PPL 显着提高了纳米蛋白质相互作用的时间分辨率至 5 秒持续时间。此外，nano-PPL 分别实现了蛋白质电晕在阴离子和阳离子纳米粒子上的快速和动态映射。最后，部署纳米 PPL 以验证快速形成的蛋白质电晕对纳米颗粒与细胞的初始相互作用的影响。这些发现突出了纳米药物在体内递送过程中纳米蛋白质相互作用的重大方法学进步。

更新日期：2022-12-07

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