With N,N′-methylenebisacrylamide (MBA) and polyvinyl alcohol (PVA) as raw materials, a polymer (PVA-MBA) containing N-halamine precursor functional groups was obtained via grafting reaction between the active hydroxyl groups on PVA and α, β-unsaturated functional groups of MBA under the catalysis of sodium carbonate in an aqueous solution. An acyclic N-halamine precursor-grafted PVA (MBA-PVA) film was formed by simply spreading PVA-MBA aqueous solution in a glass dish and drying it. An antimicrobial acyclic N-halamine-grafted PVA (PVA-MBA-Cl) film was achieved by spraying the diluted sodium hypochlorite solution onto the surface of PVA-MBA film. The performance test of PVA-MBA-Cl film under the optimal preparation conditions showed that the tensile performance and the hydrophobicity were improved, compared to the PVA film. The storage stability test indicated that the oxidative chlorine content Cl⁺ (atoms/cm²) of the as-prepared PVA-MBA-Cl film only reduced by 14.3% after storage for 9 weeks, showing that the antibacterial N-halamine functional groups in PVA-MBA-Cl film has excellent storage stability under room temperature. Antibacterial test showed that the PVA-MBA-Cl film had very strong antibacterial efficacies and could completely kill 1.28 × 10⁶ CFU/mL S. aureus and 1.89 × 10⁶ CFU/mL E. coli within 1 min. Therefore, PVA-MBA-Cl film will have more potential applications in food package.