水稻OsBSK基因家族的鉴定、进化及表达分析,BMC Plant Biology

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水稻OsBSK基因家族的鉴定、进化及表达分析
BMC Plant Biology ( IF 4.3 ) Pub Date : 2022-12-05 , DOI: 10.1186/s12870-022-03905-1
Shuo Zhang _{1,

2} , Xuewei Hu _{1,

2} , Jiejing Dong _{1,

2} , Mengxiang Du _{1,

2} , Juqi Song _{1,

2} , Shangyuan Xu _{1,

2} , Changjiang Zhao _{1,

2,

3,

4}

Affiliation

作为 BR（油菜素内酯）信号通路的重要组成部分，BSK（BR 信号激酶）在植物生长、发育和胁迫调节中起着至关重要的作用。关于该家族成员在单子叶模式植物水稻中的功能已有零星报道，但鲜有关于该家族基因的系统发育分析和基因表达谱的报道。在本研究中，通过生物信息学方法在基因组水平上共鉴定出 6 个 OsBSK 成员，分布在水稻的 4 条染色体上。通过对来自22个物种的74个BSK蛋白的进化分析，发现BSKs起源于高等植物，高度保守，可分为6个亚群。其中，OsBSKs 属于四个亚组或两个显着组。OsBSK 家族基因启动子含有大量光、脱落酸(ABA) 和茉莉酸甲酯(MeJA) 反应相关元件。同时，qRT-PCR检测也显示该家族基因参与了对多种激素、生物和非生物胁迫处理的响应，该家族基因的表达模式大致可分为两类，与基因在不同生长阶段的组织表达模式相似。OsBSK1-1、OsBSK1-2 和 OsBSK3 大部分上调。OsBSK2、OsBSK4 和 OsBSK5 大部分被下调或表达几乎没有变化。本研究揭示了 BSK 家族的起源和进化，以及 BSK 在水稻生长、发育和胁迫反应中的转移。为深入分析BR激素提供理论参考，

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Identification, evolution, and expression analysis of OsBSK gene family in Oryza sativa Japonica

As an essential component of the BR (brassinosteroid) signaling pathway, BSK (BR-signalling kinases) plays a vital role in plant growth, development, and stress regulation. There have been sporadic reports on the functions of members of this family in monocotyledonous model plant rice, but few reports have been reported on the phylogenetic analysis and gene expression profiling of the family genes. In this study, a total of 6 OsBSK members were identified at the genomic level by bioinformatics methods, distributed on four rice chromosomes. Through the evolution analysis of 74 BSK proteins from 22 species, it was found that BSKs originated from higher plants, were highly conserved, and could be divided into six subgroups. Among them, OsBSKs belonged to four subgroups or two significant groups. OsBSK family gene promoters contained a large number of light, abscisic acid (ABA), and methyl jasmonate (MeJA) response-related elements. At the same time, the qRT-PCR test also showed that the genes of this family were involved in response to a variety of hormones, biotic and abiotic stress treatments, and expression patterns of the family gene can be roughly divided into two categories, which were similar to the tissue expression patterns of genes in different growth stages. OsBSK1–1, OsBSK1–2, and OsBSK3 were mostly up-regulated. OsBSK2, OsBSK4, and OsBSK5 were mostly down-regulated or had little change in expression. This study revealed the origin and evolution of the BSK family and the farm-out of BSKs in rice growth, development, and stress response. It provides the theoretical reference for in-depth analysis of BR hormone, signal transduction, and molecular breeding design for resistance.

更新日期：2022-12-05

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