Fifteen rhenium(I) tricarbonyl complexes of the form fac-[Re(N,O’)(CO)₃(X)], where N,O’-bidentate ligand = 2-picolinic acid (Pico); 3,5-difluoropyridine-2-carboxylic acid (Dfpc); 3-trifluoromethyl-pyridine-2-carboxylic acid (Tfpc) and X = H₂O; pyrazole (Pz); pyridine (Py); imidazole (Im); and methanol (CH₃OH) were synthesized using the ‘2 + 1’ mixed ligand approach with an average yield of 84%. The complexes were characterized using the following spectroscopic techniques: IR, ¹H and ¹³C NMR, UV/Vis, and single-crystal X-ray diffraction. The effect of the fluorine atoms on the backbone of the N,O’-bidentate ligand was investigated and a trend was noticed in the carbonyl stretching frequencies: with Pico < Tfpc < Dfpc. The in vitro biological screening on Vero (healthy mammalian), HeLa (cervical carcinoma) and A549 (lung cancer) cells revealed one toxic complex, fac-[Re(Pico)(CO)₃(H₂O)], with respective LC₅₀ values of 9.0 ± 0.9, 15.8 ± 4.9 (SI = 0.570) and 20.9 ± 0.8 (SI = 0.430) μg/mL. As a result, it can be used as a positive control drug of toxicity.

fac -[Re( N,O' )(CO) ₃ (X)]形式的十五个铼 (I) 三羰基络合物，其中N,O' -双齿配体 = 2-吡啶甲酸 (Pico)；3,5-二氟吡啶-2-甲酸 (Dfpc)；3-三氟甲基-吡啶-2-甲酸(Tfpc)且X = H ₂ O；吡唑 (Pz); 吡啶 (Py)；咪唑（Im）；和甲醇 (CH ₃ OH) 是使用“2 + 1”混合配体方法合成的，平均产率为 84%。使用以下光谱技术对复合物进行表征：IR、¹ H 和¹³ C NMR、UV/Vis 和单晶 X 射线衍射。氟原子对N,O'主链的影响研究了双齿配体，发现羰基伸缩频率有一个趋势：Pico < Tfpc < Dfpc。对 Vero（健康哺乳动物）、HeLa（宫颈癌）和 A549（肺癌）细胞进行的体外生物筛选揭示了一种毒性复合物，fac-[ Re (Pico)(CO) ₃ (H ₂ O)]，以及相应的 LC 9.0 ± 0.9、15.8 ± 4.9 (SI = 0.570) 和 20.9 ± 0.8 (SI = 0.430) μg/mL 的 50 个值_。因此，它可以作为毒性的阳性对照药物。