通过与亲氧性铋合金化用于生物传感来操纵钯基纳米酶的类过氧化物酶活性,Small

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通过与亲氧性铋合金化用于生物传感来操纵钯基纳米酶的类过氧化物酶活性
Small ( IF 13.0 ) Pub Date : 2022-12-03 , DOI: 10.1002/smll.202205997
Shiyu Xia _{1,

2} , Fengxia Wu ₁ , Lu Cheng ₃ , Haibo Bao ₁ , Wenping Gao _{1,

2} , Jin Duan _{1,

2} , Wenxin Niu _{1,

2} , Guobao Xu _{1,

2}

Affiliation

设计纳米酶的催化性能对于它们在生物分析、癌症治疗和环境管理中的广泛应用至关重要。在此，展示了一种利用亲氧金属铋 (Bi) 提高 Pd 基纳米酶类过氧化物酶活性的策略，该策略基于在 Pd 基合金纳米晶体 (NCs) 中掺入亲氧性 Bi。为了合成 PdBi 合金 NCs，采用种子介导的方法，借助 Bi 在 Pd 上的欠电位沉积 (UPD)。Bi原子与Pd表面的强相互作用有利于形成具有可控形状和优异单分散性的合金结构。更重要的是，与原始 Pd NC 相比，PdBi NC 表现出优异的过氧化物酶样活性。阐明了 PdBi 纳米酶的结构-功能相关性，并建立了一种基于级联反应的间接比色法来测定碱性磷酸酶 (ALP)。该方法线性范围好、检出限低、选择性好、抗干扰性好。总的来说，这项工作不仅为高效纳米酶的设计提供了新的见解，扩展了基于酶级联反应的比色传感平台，而且代表了合金 NCs 的 UPD 定向合成的新实例。

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Maneuvering the Peroxidase-Like Activity of Palladium-Based Nanozymes by Alloying with Oxophilic Bismuth for Biosensing

Engineering the catalytic performance of nanozymes is of vital importance for their broad applications in biological analysis, cancer treatment, and environmental management. Herein, a strategy to boost the peroxidase-like activity of Pd-based nanozymes with oxophilic metallic bismuth (Bi) is demonstrated, which is based on the incorporation of oxophilic Bi in the Pd-based alloy nanocrystals (NCs). To synthesize PdBi alloy NCs, a seed-mediated method is employed with the assistance of underpotential deposition (UPD) of Bi on Pd. The strong interaction of Bi atoms with Pd surfaces favors the formation of alloy structures with controllable shapes and excellent monodispersity. More importantly, the PdBi NCs show excellent peroxidase-like activities compared with pristine Pd NCs. The structure–function correlations for the PdBi nanozymes are elucidated, and an indirect colorimetric method based on cascade reactions to determine alkaline phosphatase (ALP) is established. This method has good linear range, low detection limit, excellent selectivity, and anti-interference. Collectively, this work not only provides new insights for the design of high-efficiency nanozymes, expands the colorimetric sensing platform based on enzyme cascade reactions, but also represents a new example for UPD-directed synthesis of alloy NCs.

更新日期：2022-12-03

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