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Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops
Scientific Data ( IF 5.8 ) Pub Date : 2022-12-03 , DOI: 10.1038/s41597-022-01859-5
Mengdi Zhang 1 , Xinyue Cheng 2 , Runmao Lin 3, 4 , Bingyan Xie 3 , Ralf Nauen 5 , Silvia I Rondon 6 , Jorge A Zavala 7 , Subba Reddy Palli 8 , Suhua Li 9 , Xingyao Xiong 9 , Wenwu Zhou 10 , Yulin Gao 1, 11
Affiliation  

The potato tuberworm, Phthorimaea operculella Zeller, is an oligophagous pest feeding on crops mainly belonging to the family Solanaceae. It is one of the most destructive pests of potato worldwide and attacks foliage and tubers in the field and in storage. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis. Here, we report on the genome assembly of P. operculella at the chromosomal level. Using Illumina, Nanopore and Hi-C sequencing, a 648.2 Mb genome was generated from 665 contigs, with an N50 length of 3.2 Mb, and 92.0% (596/648.2 Mb) of the assembly was anchored to 29 chromosomes. In total, 16619 genes were annotated, and 92.4% of BUSCO genes were fully represented. The chromosome-level genome of P. operculella will provide a significant resource for understanding the genetic basis for the biological study of this insect, and for promoting the integrative management of this pest in future.



中文翻译:


马铃薯块茎虫 (Phthorimaea operculella) 的染色体水平基因组组装:茄科作物害虫



马铃薯块茎虫( Phthorimaea operculella Zeller)是一种寡食性害虫,主要以茄科作物为食。它是全世界马铃薯最具破坏性的害虫之一,危害田间和储藏室的叶子和块茎。然而,缺乏高质量的参考基因组阻碍了表型性状与其遗传基础的关联。在这里,我们报告了P的基因组组装。染色体水平上的operculella 。使用 Illumina、Nanopore 和 Hi-C 测序,从 665 个重叠群生成了 648.2 Mb 的基因组,N50 长度为 3.2 Mb,并且 92.0% (596/648.2 Mb) 的组装锚定到 29 条染色体。总共注释了16619个基因,充分代表了92.4%的BUSCO基因。 P的染色体水平基因组。 operculella将为了解该昆虫的生物学研究的遗传基础以及促进未来对该害虫的综合治理提供重要资源。

更新日期:2022-12-03
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