A cytochrome P450 2D6 (CYP2D6) biosensor was developed with the incorporation of multi-walled carbon nanotube/ionic liquid and tetrathiafulvalene-tetracyanoquinodimethane salt/ionic liquid (MWNT/IL-[TTF–TCNQ]/IL) gels. The MWNT/IL-[TTF–TCNQ]/IL gels were characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, and scanning electron microscopy. Utilizing the excellent stability of the MWNT/IL gel and the unique structure of the [TTF–TCNQ]/IL gel, CYP2D6 was immobilized on the screen-printed electrode with high bioactivity. Moreover, the CYP2D6 catalytic effect was highly promoted upon application of MWNT/IL-[TTF–TCNQ]/IL gels as the mediating system. The proposed MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor was used to detect perphenazine via voltammetry and amperometry. The calibration of perphenazine ranged from 6 × 10⁻⁸ to 1.6 × 10⁻⁵ M with a detection limit of 2.05 × 10⁻⁸ M. It is expected that the MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor opens new avenues for promising advances in the analysis of drugs.

开发了一种细胞色素 P450 2D6 (CYP2D6) 生物传感器，结合了多壁碳纳米管/离子液体和四硫富瓦烯-四氰基喹啉二甲烷盐/离子液体 (MWNT/IL-[TTF–TCNQ]/IL) 凝胶。MWNT/IL-[TTF–TCNQ]/IL 凝胶使用傅立叶变换红外光谱、紫外-可见光谱和扫描电子显微镜进行了表征。利用 MWNT/IL 凝胶优异的稳定性和 [TTF–TCNQ]/IL 凝胶的独特结构，将 CYP2D6 固定在具有高生物活性的丝网印刷电极上。此外，在应用 MWNT/IL-[TTF–TCNQ]/IL 凝胶作为中介系统后，CYP2D6 的催化作用得到了极大的促进。所提出的基于 MWNT/IL-[TTF–TCNQ]/IL 凝胶的 CYP2D6 生物传感器用于通过伏安法和安培法检测奋乃静。⁻⁸至 1.6 × 10 ⁻⁵  M，检测限为 2.05 × 10 ⁻⁸  M。预计基于 MWNT/IL-[TTF–TCNQ]/IL 凝胶的 CYP2D6 生物传感器开辟了新途径，有望在药物分析。