Immobilization of lipase AK from Pseudomonas fluorescens onto a microsphere support constructed by the metal organic framework (MOF) of NH₂-Co-MOF was investigated. The loading capacity of 256.9 mg/g (MOF) with an immobilization yield of 72.3% was achieved. The further investigation demonstrated that AK@NH₂-Co-MOF was an efficient catalyst in enantiospecific transesterification of 1-(4-bromophenyl) ethanol (BPE) with vinyl acetate, where the expressed activity of (4.028 ± 0.21)× 10⁻² mmol/(min·mg AK@NH₂-Co-MOF) was 8 times higher than the free lipase. Under the optimum conditions, BPE enantiomers were resolved by AK@NH₂-Co-MOF with conversion of 50.42%, enantiomeric excess > 99% for remaining substrate (ee_s) and enantiomeric excess > 99 % for product (ee_p). AK@NH₂-Co-MOF is recyclable and 51.62 % of the initial conversion was retained after 5 cycles of reuse. NH₂-Co-MOF is promising for enzyme immobilization and the prepared AK@NH₂-Co-MOF exhibited great potential for efficient biosynthesis of optically pure BPE.

研究了将来自荧光假单胞菌的脂肪酶 AK 固定到由 NH ₂ -Co-MOF的金属有机骨架 (MOF) 构建的微球载体上。负载量为 256.9 mg/g (MOF)，固定化率为 72.3%。进一步研究表明，AK@NH ₂ -Co-MOF是1-(4-溴苯基)乙醇(BPE)与乙酸乙烯酯的对映特异性酯交换反应的有效催化剂，其活性为(4.028 ± 0.21)× 10 ^-2 mmol/(min·mg AK@NH ₂ -Co-MOF)比游离脂肪酶高8倍。在最佳条件下，BPE对映异构体被AK@NH _2拆分-Co-MOF，转化率为 50.42%，剩余底物 ( ee _s ) 的对映体过量 > 99%，产物 ( ee _p ) 的对映体过量 > 99%。AK@NH ₂ -Co-MOF 是可回收的，51.62% 的初始转化率在 5 次循环再利用后得以保留。NH ₂ -Co-MOF有望用于酶固定化，所制备的AK@NH ₂ -Co-MOF在高效生物合成光学纯BPE方面表现出巨大潜力。