用于生物微环境检测的液态金属pH形貌传感器,Analytical Chemistry

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用于生物微环境检测的液态金属pH形貌传感器
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-11-29 , DOI: 10.1021/acs.analchem.2c04357
Yang Wang ₁ , Liangtao Li ₁ , Shuting Liang ₁ , Kang Sun ₁ , Caicai Jiao ₁ , Qian Wang ₁ , Liang Hu ₁

Affiliation

pH值是生物微环境的重要参数之一，与细胞的生长、发育、活力、分裂和分化密切相关。监测微环境的 pH 值有助于监测细胞代谢以及了解细胞生命周期。液态金属 (LM) 对氢离子的敏感性引起了我们的兴趣。在这里，我们提出了一种新颖但简便的 pH 传感器，使用液态镓（简称 LM）液滴形态变化作为读数。检查了 LM 液滴的 pH 传感特性，尤其是形状响应。LM在含氧溶液中能迅速形成固态的天然氧化皮，氧化层在酸性或碱性溶液中会被去除，这会引起表面张力的巨大变化。这种现象是宏观观察到的LM形态的变化。我们探索了LM在不同pH值下的电化学特性，解释了表面变化的机理，并标定了LM形貌与pH和杂质离子对传感器干扰的关系曲线。最后，我们提出了LM pH形态传感器的检测算法，并尝试通过手机APP自动检测pH值，应用于细胞培养液的pH值检测。我们认为LM对氢离子的响应特性在微环境检测中具有巨大的潜力。标定了LM形貌与pH值及杂质离子对传感器干扰的关系曲线。最后，我们提出了LM pH形态传感器的检测算法，并尝试通过手机APP自动检测pH值，应用于细胞培养液的pH值检测。我们认为LM对氢离子的响应特性在微环境检测中具有巨大的潜力。标定了LM形貌与pH值及杂质离子对传感器干扰的关系曲线。最后，我们提出了LM pH形态传感器的检测算法，并尝试通过手机APP自动检测pH值，应用于细胞培养液的pH值检测。我们认为LM对氢离子的响应特性在微环境检测中具有巨大的潜力。

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Liquid Metal pH Morphology Sensor Used for Biological Microenvironment Detection

pH is one of the important parameters of a biological microenvironment, which is closely related to cell growth, development, vitality, division, and differentiation. Monitoring the pH of a microenvironment is helpful to monitor the cell metabolism as well as to understand the cellular life cycle. The sensitivity of liquid metals (LMs) to hydrogen ions has aroused our interest. Here, we propose a novel but facile pH sensor using liquid gallium (LM for short) droplet morphological change as the readout. The pH sensing characteristics of the LM droplet were examined, especially the shape response. LM can form solid native oxide skin rapidly in oxygenated solution, and the oxide layer will be removed in acidic or alkaline solutions, which will cause a great change in surface tension. The phenomenon is the change of LM morphology from macroscopic observation. We explored the electrochemical characteristics of LM at different pH values, explained the mechanism of surface change, and calibrated the relationship curve between LM morphology and pH and the interference of impurity ions on the sensor. Finally, we proposed a detection algorithm for the LM pH morphology sensor and tried to automatically detect pH with a mobile app, which was applied to the pH detection of cell culture solution. We believe that the response characteristics of LM to hydrogen ions have great potential in microenvironment detection.

更新日期：2022-11-29

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