Streptococcus agalactiae cadD Is Critical for Pathogenesis in the Invertebrate Galleria mellonella Model,ACS Infectious Diseases

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Streptococcus agalactiae cadD Is Critical for Pathogenesis in the Invertebrate Galleria mellonella Model
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2022-11-29 , DOI: 10.1021/acsinfecdis.2c00453
Miriam A Guevara ₁ , Jamisha D Francis ₁ , Jacky Lu ₁ , Shannon D Manning ₂ , Ryan S Doster ₃ , Rebecca E Moore ₁ , Jennifer A Gaddy _{1,

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Affiliation

Group B Streptococcus (GBS) is a gram-positive bacterium that can cause invasive infections in immunocompromised, elderly, pregnant, or neonatal patients. The invertebrate model, Galleria mellonella, has emerged as an effective tool to study GBS-host interactions; specifically, those conserved within the innate arm of the immune system. We sought to determine the role of metal homeostasis functions in GBS infections of G. mellonella larvae and to validate this model as a tool to study GBS-host interactions. Our results indicate that wild-type GBS infects G. mellonella in a dose-dependent manner, replicates in the invertebrate host, induces larval melanization and larval killing. These results were significantly abrogated in cohorts of larvae infected with the isogenic cadD deletion mutant. Additionally, complementation restored GBS-dependent infection, bacterial burden, larval melanization, and killing to wild-type levels. Together, these results indicate that the G. mellonella model is a useful tool for studying GBS pathogenesis.

中文翻译：

无乳链球菌 cadD 对于无脊椎动物大蜡螟模型的发病机制至关重要

B 族链球菌(GBS) 是一种革兰氏阳性细菌，可引起免疫功能低下、老年人、孕妇或新生儿患者的侵袭性感染。无脊椎动物模型大蜡螟已成为研究 GBS 与宿主相互作用的有效工具；具体来说，是那些在免疫系统先天臂内保守的。我们试图确定金属稳态功能在大蜡螟幼虫 GBS 感染中的作用，并验证该模型作为研究 GBS-宿主相互作用的工具。我们的结果表明，野生型GBS以剂量依赖性方式感染大蜡螟，在无脊椎动物宿主中复制，诱导幼虫黑色化和幼虫死亡。这些结果在感染同基因cadD缺失突变体的幼虫群体中显着消失。此外，互补将 GBS 依赖性感染、细菌负担、幼虫黑化和杀灭恢复到野生型水平。总之，这些结果表明大蜡螟模型是研究 GBS 发病机制的有用工具。

更新日期：2022-11-29

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