通过与 DNA 光修复所需蛋白质连接的 Rad1 和 Rad10 对太阳紫外线损伤的绿僵菌进行高效光再激活,Photochemistry and Photobiology

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通过与 DNA 光修复所需蛋白质连接的 Rad1 和 Rad10 对太阳紫外线损伤的绿僵菌进行高效光再激活
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2022-11-28 , DOI: 10.1111/php.13752
Yi‐Lu Zhang ₁ , Han Peng ₁ , Sheng‐Hua Ying ₁ , Ming‐Guang Feng ₁

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紫外线 (UV) 诱导的 DNA 损伤（称为环丁烷嘧啶二聚体 (CPD) 和 (6-4)-嘧啶-嘧啶酮 (6-4PP) 光产物）的核苷酸切除修复 (NER) 取决于多种抗紫外线辐射的活性。 RAD）芽殖酵母中的蛋白质。然而，在丝状真菌中，NER 仍然鲜为人知，丝状真菌的 DNA 损伤是通过一种或两种光裂合酶（即 CPD 特异性 Phr1 和/或 6-4PP 特异性 Phr2）进行光修复的。此前，白领蛋白 WC1 和 WC2 被证明可分别调节罗伯茨绿僵菌中phr2和phr1的表达以及光修复 6-4PP 和 CDP DNA 损伤，一种丝状昆虫病原植物内生真菌。我们在这里报道了直系同源 Rad1 和 Rad10 在 UVB 损伤或 UVB 灭活分生孢子的 5 小时光再激活中的高活性，但它们通过 24 小时黑暗孵育在M. robertsii 中重新激活这些分生孢子的能力严重受损。与之前的phr1、phr2、wc1和wc2无效突变体相比， rad1和rad10的无效突变体在分生孢子 UVB 抗性和光再激活能力方面受到更大的损害。。检测到的多种蛋白质-蛋白质（Rad1-Rad10、Rad1-WC2、Rad10-Phr1、WC1-Phr1/2 和 WC2-Phr1/2）相互作用表明 Rad1 和 Rad10 与 Phr1/2 和 WC1/2 存在直接/间接联系，并且这些链接对于其光活化活动的重要性。总之，Rad1 和 Rad10通过与 DNA 光修复所需蛋白质的相互作用，对UVB 损伤的罗伯氏分枝杆菌进行光再激活。

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Efficient Photoreactivation of Solar UV-Injured Metarhizium robertsii by Rad1 and Rad10 Linked to DNA Photorepair-Required Proteins

Nucleotide excision repair (NER) of ultraviolet (UV)-induced DNA lesions known as cyclobutane pyrimidine dimer (CPD) and (6–4)-pyrimidine-pyrimidone (6-4PP) photoproducts depends on the activities of multiple anti-UV radiation (RAD) proteins in budding yeast. However, NER remains poorly known in filamentous fungi, whose DNA lesions are photorepaired by one or two photolyases, namely CPD-specific Phr1 and/or 6-4PP-specific Phr2. Previously, the white collar proteins WC1 and WC2 were proven to regulate expressions of phr2 and phr1 and photorepair 6-4PP and CDP DNA lesions, respectively, in Metarhizium robertsii, a filamentous entomopathogenic-phytoendophytic fungus. We report here high activities of orthologous Rad1 and Rad10 in 5-h photoreactivation of UVB-injured or UVB-inactivated conidia but a severely compromised capability of their reactivating those conidia via 24-h dark incubation in M. robertsii. The null mutants of rad1 and rad10 were much more compromised in conidial UVB resistance and photoreactivation capability than the previous null mutants of phr1, phr2, wc1 and wc2. Multiple protein–protein (Rad1-Rad10, Rad1-WC2, Rad10-Phr1, WC1-Phr1/2 and WC2-Phr1/2) interactions detected suggest direct/indirect links of Rad1 and Rad10 to Phr1/2 and WC1/2 and an importance of the links for their photoreactivation activities. Conclusively, Rad1 and Rad10 photoreactivate UVB-impaired M. robertsii through their interactions with the DNA photorepair-required proteins.

更新日期：2022-11-28

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