Brucellosis is a chronic infectious disease caused by Brucella, which is characterized by inflammation of reproductive organs and fetal membranes, abortion, infertility, and local inflammatory lesions of various tissues. Due to the widespread prevalence and spread of brucellosis, it has not only caused huge losses to animal husbandry, but also brought serious impacts on human health and safety. Therefore, rapid and accurate diagnosis is of great significance for the effective control of brucellosis. Therefore, we have developed a rapid vertical flow technique (RVFT) using Prussian blue nanoparticles (PBNPs) as a marker material for the detection of brucellosis antibodies. Lipopolysaccharide (LPS) was purified and used to detect brucellosis antibodies to improve the sensitivity of this technique. To enhance the sensitivity of serum antibody detection, a single multifunctional compound buffer was created using whole blood as a biological sample while retaining the advantages of typical lateral flow immunoassays. After signal amplification, standard Brucella-positive serum (containing Brucella antibody at 4000 IU mL⁻¹) could be detected in this system even at a dilution factor of 1 × 10⁻². The detection limit was 40 IU mL⁻¹, which is ten times that before signal amplification. This RVFT displayed good specificity and no cross-reactivity. This RVFT effectively avoided the false negative phenomenon of lateral flow immunoassays, was easy to operate, had a short reaction time, has good repeatability, and could elicit results that were visible to the naked eye for 2 ~ 3 min without any equipment. Since this method is very important for controlling the prevalence of brucellosis, it holds great promise for application in primary medical units and veterinary brucellosis detection.

布鲁氏菌病是由布鲁氏菌引起的慢性传染病以生殖器官及胎膜炎症、流产、不育及各组织局部炎性病变为特征。由于布鲁氏菌病的广泛流行和传播，不仅给畜牧业造成巨大损失，也给人类健康和生命安全带来严重影响。因此，快速、准确的诊断对于有效控制布鲁氏菌病具有重要意义。因此，我们开发了一种使用普鲁士蓝纳米颗粒（PBNPs）作为检测布鲁氏菌病抗体的标记材料的快速垂直流技术（RVFT）。纯化脂多糖 (LPS) 并用于检测布鲁氏菌病抗体，以提高该技术的灵敏度。为提高血清抗体检测的灵敏度，使用全血作为生物样本创建了单一的多功能复合缓冲液，同时保留了典型横向流动免疫测定的优点。信号放大后，标准^{即使在 1 × 10 -2}的稀释倍数下，也可以在该系统中检测到布鲁氏菌阳性血清（含有4000 IU mL ^-1的布鲁氏菌抗体） 。检测限为 40 IU mL ^-1，是信号放大前的十倍。此 RVFT 显示出良好的特异性且无交叉反应性。该RVFT有效避免了侧流免疫检测的假阴性现象，操作简单，反应时间短，重复性好，无需任何设备即可引出肉眼可见的结果2~3 min。由于该方法对于控制布鲁氏菌病的流行非常重要，因此在基层医疗单位和兽医布鲁氏菌病检测中具有广阔的应用前景。