International Journal of Peptide Research and Therapeutics ( IF 2.0 ) Pub Date : 2022-11-24 , DOI: 10.1007/s10989-022-10473-3 Liang Wei , Hui Li , Xinxin Yu , Huiying Yang , Duowen Pu , Minghua Zhu , Qi Lu , Yihong Bao , Yuangang Zu
As a new food raw material, Acer truncatum seed has been used in the production of edible oil. However, the research on the processing residue (seed meal) has not been reported. The Acer truncatum seed meal was hydrolyzed by seven enzymes and the enzymic polypeptides obtained were characterized by thermogravimetric analysis, gel permeation chromatography, fourier transform infrared spectroscopy and zeta pals dynamic light scattering. In addition, the degree of hydrolysis, amino acid composition, antioxidant activity, α-glucosidase inhibition activity and α-amylase inhibition activity of enzymatic polypeptide were evaluated. The results showed that the yields, average molecular weights, average particle sizes and zeta potentials of enzymic polypeptides obtained were 20.5–40.2%, 7.8–13.9 kDa, 288.3–997.5 nm and ( −)1.8 to ( −)36.4 mV, respectively. The bioactivity results showed that the enzymatic polypeptides not only had higher antioxidant activity, but also better α-glucosidase and α-amylase inhibition activities than the raw materials. Among the seven enzymatic polypeptides, the alkaline protease enzymatic polypeptide had the highest content of the eight essential amino acids (20.3%), the largest value of hydrolysis degree (15.4%) and the most significant bioactivity. The study will provide the data support for developing the new application of seed meal from Acer truncatum.
中文翻译:
元宝枫籽粕酶多肽的氨基酸组成、抗氧化、α-葡萄糖苷酶和α-淀粉酶抑制活性
元宝枫籽作为一种新型食品原料,已被用于食用油的生产。但对加工残渣(籽粕)的研究尚未见报道。元宝枫_种子粉被七种酶水解,所获得的酶促多肽通过热重分析、凝胶渗透色谱、傅里叶变换红外光谱和 zeta pals 动态光散射进行了表征。此外,还评价了酶促多肽的水解程度、氨基酸组成、抗氧化活性、α-葡萄糖苷酶抑制活性和α-淀粉酶抑制活性。结果表明,所得酶促多肽的产率、平均分子量、平均粒径和zeta电位分别为20.5-40.2%、7.8-13.9 kDa、288.3-997.5 nm和(-)1.8-(-)36.4 mV。生物活性结果表明,酶促多肽不仅具有比原料更高的抗氧化活性,而且对α-葡萄糖苷酶和α-淀粉酶的抑制活性也更好。在七种酶促多肽中,碱性蛋白酶酶促多肽八种必需氨基酸含量最高(20.3%),水解度值最大(15.4%),生物活性最显着。该研究将为开发种子粕的新应用提供数据支持。元宝枫。