<br>NIR-II 窗口中的深度分辨定位微血管造影,Advanced Science

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NIR-II 窗口中的深度分辨定位微血管造影
Advanced Science ( IF 14.3 ) Pub Date : 2022-11-20 , DOI: 10.1002/advs.202204782
Quanyu Zhou _{1,

2} , Daniil Nozdriukhin _{1,

2} , Zhenyue Chen _{1,

2} , Lukas Glandorf _{1,

2} , Urs A T Hofmann _{1,

2} , Michael Reiss _{1,

2} , Lin Tang _{1,

2} , Xosé Luís Deán-Ben _{1,

2} , Daniel Razansky _{1,

2}

Affiliation

微血管改变的详细表征需要能够提供形态和功能信息的高分辨率 3D 成像方法。现有的光学显微镜工具通常用于微血管造影，但在可实现的视野和空间分辨率之间提供了次优的权衡，生物组织中的强光散射进一步限制了可实现的穿透深度。本文介绍了一种基于立体视觉与超分辨率定位成像相结合的体积深层组织微血管造影的新方法，该方法克服了宽视场成像配置中光扩散和光学衍射所带来的空间分辨率限制。该方法利用第二个近红外窗口（NIR-II，约 1000–1700 nm）中流动荧光粒子的定位和跟踪，通过三角测量和对用两个短波长采集的图像进行立体匹配来添加第三（深度）维度。 -以双视图模式运行的wave红外摄像机。通过所提出的方法实现的 3D 成像功能有助于微血管网络的详细可视化和准确的血流量化。在模拟组织模型中进行的实验表明，在浑浊介质中深度达 4 毫米的情况下仍能保持高分辨率。整个小鼠皮质和穿透血管的经颅微血管造影在毛细血管水平分辨率上得到进一步证明。

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Depth-Resolved Localization Microangiography in the NIR-II Window

Detailed characterization of microvascular alterations requires high-resolution 3D imaging methods capable of providing both morphological and functional information. Existing optical microscopy tools are routinely used for microangiography, yet offer suboptimal trade-offs between the achievable field of view and spatial resolution with the intense light scattering in biological tissues further limiting the achievable penetration depth. Herein, a new approach for volumetric deep-tissue microangiography based on stereovision combined with super-resolution localization imaging is introduced that overcomes the spatial resolution limits imposed by light diffusion and optical diffraction in wide-field imaging configurations. The method capitalizes on localization and tracking of flowing fluorescent particles in the second near-infrared window (NIR-II, ≈1000–1700 nm), with the third (depth) dimension added by triangulation and stereo-matching of images acquired with two short-wave infrared cameras operating in a dual-view mode. The 3D imaging capability enabled with the proposed method facilitates a detailed visualization of microvascular networks and an accurate blood flow quantification. Experiments performed in tissue-mimicking phantoms demonstrate that high resolution is preserved up to a depth of 4 mm in a turbid medium. Transcranial microangiography of the entire murine cortex and penetrating vessels is further demonstrated at capillary level resolution.

更新日期：2022-11-20

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